Dadar Maryam, Alamian Saeed, Tadayon Keyvan, Ashford Roland T, Whatmore Adrian M
Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Acta Trop. 2022 May;229:106363. doi: 10.1016/j.actatropica.2022.106363. Epub 2022 Feb 9.
Brucellosis is an endemic infection in Iran and represents a serious health problem in humans and livestock causing important economic losses. The objective of this study was to undertake molecular characterization of Brucella spp. isolated from humans and livestock in several provinces of Iran including by multi-locus sequence typing (MLST), in order to understand the genotypes circulating in Iran and their relationship to genotypes globally. A total of 23 Brucella isolates were isolated from eight milk samples (seven cows, and one camel), human blood samples (seven), bovine lymph nodes (two), and samples from aborted fetuses (three sheep, two cows, and one goat). Phenotypic and molecular identification of Brucella isolates was performed on all isolated bacteria and showed that all were either Brucella melitensis or Brucella abortus. B. melitensis was associated with ovine/caprine and camel samples, most human isolates, and a significant minority of cattle isolates. In contrast B. abortus from livestock was associated only with isolations from bovine samples, as well as a single human sample. These results indicate that both B. melitensis and B. abortus contribute to the human brucellosis burden in Iran. B. melitensis isolates comprised three MLST-9 genotypes, the common and globally distributed ST8, a single representative of ST7, and several additional examples of ST102, a genotype previously only reported in a single isolate from a human brucellosis case believed to be acquired through travel to Iran. B. abortus isolates represented two globally common MLST-9 genotypes (ST1 and ST2), with relationships to biotype and other PCR-based typing methods consistent with previous observations. The results provide the basis for further studies examining the molecular epidemiology of Brucella circulating in Iran and the relationships of local isolates to those present globally.
布鲁氏菌病在伊朗是一种地方感染病，对人类和牲畜来说是一个严重的健康问题，会造成重大经济损失。本研究的目的是对从伊朗多个省份的人类和牲畜中分离出的布鲁氏菌进行分子特征分析，包括通过多位点序列分型（MLST），以了解在伊朗流行的基因型及其与全球基因型的关系。总共从八个牛奶样本（七头牛和一头骆驼）、人类血液样本（七个）、牛淋巴结（两个）以及流产胎儿样本（三只羊、两头牛和一只山羊）中分离出23株布鲁氏菌。对所有分离出的细菌进行了布鲁氏菌的表型和分子鉴定，结果表明所有菌株均为羊种布鲁氏菌或牛种布鲁氏菌。羊种布鲁氏菌与绵羊/山羊和骆驼样本、大多数人类分离株以及少数牛分离株有关。相比之下，牲畜中的牛种布鲁氏菌仅与牛样本以及一个人类样本的分离有关。这些结果表明，羊种布鲁氏菌和牛种布鲁氏菌都对伊朗人类布鲁氏菌病负担有影响。羊种布鲁氏菌分离株包括三种MLST-9基因型，常见且全球分布的ST8、ST7的单一代表以及ST102的几个其他实例，ST102基因型此前仅在一例被认为是通过前往伊朗感染的人类布鲁氏菌病病例的单一分离株中报道过。牛种布鲁氏菌分离株代表两种全球常见的MLST-9基因型（ST1和ST2），其与生物型和其他基于PCR的分型方法的关系与先前观察结果一致。这些结果为进一步研究伊朗流行的布鲁氏菌分子流行病学以及当地分离株与全球现有分离株的关系提供了基础。
