Doria M I, Montag A G, Franklin W A
Section of Surgical Pathology, University of Chicago, Illinois 60637.
Cancer. 1988 Nov 1;62(9):1939-45. doi: 10.1002/1097-0142(19881101)62:9<1939::aid-cncr2820620912>3.0.co;2-n.
NKH-1 is a monoclonal antibody that reacts with human natural killer (NK) cells and neural tissue. Because other monoclonal antibodies reacting with NK cells have been found on small cell lung carcinoma (SCLC), frozen tissue sections of 22 lung tumors including nine SCLC, two bronchial carcinoids, and 11 non-SCLC were tested for the presence of NKH-1 antigen by a sensitive alkaline phosphatase/anti-alkaline phosphatase technique. The labeling reactions of NKH-1 in frozen tissue sections were compared with reactions of a panel of 21 other monoclonal antibodies against NK cells, leukocyte antigens, cytokeratins, or nonlineage specific antigens. The antibody NKH-1 reacted strongly and diffusely with all of the SCLC and bronchial carcinoids but with none of the non-SCLC. NKH-1 also strongly labeled peripheral nerves in tissues adjacent to tumor. Two antibodies to cytokeratins reacted with all of the tumors and outlined tumor cells well, distinguishing them from surrounding stromal cells and leukocytes. OKT9, an antibody against transferrin receptor labeled all SCLC and eight of 11 non-SCLC but did not react with bronchial carcinoid. The antibodies Leu-M1, OKT10, Leu-7, and My4 reacted with 67%, 33%, 22%, and 11%, respectively, of the SCLC tested. The remaining 14 antibodies, including several with leukocyte specificity, labeled neither SCLC nor bronchial carcinoid. Thus, SCLC has a distinct immunophenotype (NKH-1 positive, keratin positive, and transferrin receptor positive), which may be helpful distinguishing this tumor from other tumors of lung including non-SCLC. SCLC infrequently expresses other leukocyte-associated antigens.
NKH - 1是一种单克隆抗体，可与人自然杀伤（NK）细胞及神经组织发生反应。由于在小细胞肺癌（SCLC）中发现了其他与NK细胞反应的单克隆抗体，运用灵敏的碱性磷酸酶/抗碱性磷酸酶技术，对22例肺肿瘤的冰冻组织切片进行检测，这些肺肿瘤包括9例SCLC、2例支气管类癌和11例非SCLC，以确定NKH - 1抗原的存在情况。将NKH - 1在冰冻组织切片中的标记反应，与一组针对NK细胞、白细胞抗原、细胞角蛋白或非谱系特异性抗原的21种其他单克隆抗体的反应进行比较。抗体NKH - 1与所有的SCLC和支气管类癌均发生强烈且弥漫性反应，但与非SCLC均无反应。NKH - 1还强烈标记肿瘤邻近组织中的周围神经。两种细胞角蛋白抗体与所有肿瘤均发生反应，能很好地勾勒出肿瘤细胞，将其与周围的基质细胞和白细胞区分开来。OKT9，一种抗转铁蛋白受体的抗体，标记了所有的SCLC以及11例非SCLC中的8例，但不与支气管类癌发生反应。抗体Leu - M1、OKT10、Leu - 7和My4分别与所检测SCLC的67%、33%、22%和11%发生反应。其余14种抗体，包括几种具有白细胞特异性的抗体，既不标记SCLC也不标记支气管类癌。因此，SCLC具有独特的免疫表型（NKH - 1阳性、角蛋白阳性和转铁蛋白受体阳性），这可能有助于将该肿瘤与包括非SCLC在内的其他肺肿瘤区分开来。SCLC很少表达其他白细胞相关抗原。
