从一名小细胞肺癌患者建立的人类T细胞白血病病毒1型阳性细胞系。
Human T-cell leukemia virus-1-positive cell line established from a patient with small cell lung cancer.
作者信息
Matsuzaki H, Hata H, Asou N, Yoshida M, Matsuno F, Takeya M, Yamaguchi K, Sanada I, Takatsuki K
机构信息
Second Department of Internal Medicine, Kumamoto University Medical School.
出版信息
Jpn J Cancer Res. 1992 May;83(5):450-7. doi: 10.1111/j.1349-7006.1992.tb01949.x.
A stable cell line, KHM-3S, was established from a patient with small cell lung cancer (SCLC), who had a high serum level of soluble interleukin 2 receptors (sIL2-R) and was seropositive for human T cell leukemia virus (HTLV)-1. KHM-3S cells were positive for IL2-R (Tac) and NKH-1, but negative for other lymphocytic markers such as OKT 11, OKT 4, OKT 8, T cell receptor (WT 31), B 1, and B 4. Moreover, the KHM-3S cells were negative for leukocyte common antigen and strongly positive for neuron-specific enolase (NSE). Secretion of sIL2-R and NSE by the KHM-3S line was detected by an enzyme-linked immunosorbent assay. Rearrangement of the T cell receptor gene and monoclonal HTLV-1 integration were found by Southern blot analysis of KHM-3S DNA. However, Northern blot analysis showed no T cell receptor mRNA. KHM-3S may be useful for studies on the role of HTLV-1 in carcinogenesis and IL2-R expression in SCLC.
从一名血清可溶性白细胞介素2受体（sIL2-R）水平高且人类T细胞白血病病毒（HTLV）-1血清学阳性的小细胞肺癌（SCLC）患者中建立了稳定细胞系KHM-3S。KHM-3S细胞IL2-R（Tac）和NKH-1呈阳性，但对其他淋巴细胞标志物如OKT 11、OKT 4、OKT 8、T细胞受体（WT 31）、B 1和B 4呈阴性。此外，KHM-3S细胞白细胞共同抗原呈阴性，神经元特异性烯醇化酶（NSE）呈强阳性。通过酶联免疫吸附测定检测到KHM-3S细胞系分泌sIL2-R和NSE。通过对KHM-3S DNA的Southern印迹分析发现了T细胞受体基因重排和单克隆HTLV-1整合。然而，Northern印迹分析未显示T细胞受体mRNA。KHM-3S可能有助于研究HTLV-1在致癌作用中的作用以及SCLC中IL2-R的表达。
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