Bistoletti P, Zellbi A, Moreno-Lopez J, Hjerpe A
Department of Obstetrics and Gynaecology, Karolinska Institutet, Huddinge University Hospital, Sweden.
Cancer. 1988 Nov 1;62(9):2056-9. doi: 10.1002/1097-0142(19881101)62:9<2056::aid-cncr2820620931>3.0.co;2-k.
Genital human papillomavirus (HPV) infection was studied in 150 women after conization for cervical intraepithelial neoplasia grade three (CIN III). Colposcopically directed biopsies were taken from the cervix and vulva for histopathological diagnosis. 77 specimens were further analyzed immunohistochemically for the presence of HPV capsid antigen. In ten randomly selected cervical biopsies cellular DNA was dot blot hybridized with HPV 6/11 and 16/18 DNA probes. Genital warts were seen in 10 (7%) of the patients. Among the routine cytological smears, HPV infection was only reported in 3 (2%). In 87/142 (61%) of the cervical tissues koilocytes were found. A further 9/142 (6%) associated with CIN. Of the vulvar biopsies 91/145 (63%) contained koilocytes. A further 12/145 (8%) were associated with vulvar intraepithelial neoplasia. HPV capsid proteins were detected in 35/77 (45%) of the cervical and in 20/41 (49%) of the vulva biopsies. All cervical DNA samples hybridized with probes for HPV 6/11 and two also reacted with the HPV 16/18 probes.
A latent HPV infection of the cervix or vulva, can be detected in 85% of the women previously treated for CIN III by conization.
对150例因宫颈上皮内瘤变三级（CIN III）行锥切术的女性进行了生殖道人乳头瘤病毒（HPV）感染研究。在阴道镜引导下从宫颈和外阴取活检组织进行组织病理学诊断。对77份标本进一步进行免疫组织化学分析以检测HPV衣壳抗原的存在。在随机选取的10份宫颈活检组织中，细胞DNA与HPV 6/11和16/18 DNA探针进行斑点杂交。10例（7%）患者出现尖锐湿疣。在常规细胞学涂片检查中，仅3例（2%）报告有HPV感染。在142份宫颈组织中的87份（61%）发现了挖空细胞。另外9份（6%）与CIN相关。在外阴活检组织中，145份中的91份（63%）含有挖空细胞。另外12份（8%）与外阴上皮内瘤变相关。在77份宫颈活检组织中的35份（45%）以及41份外阴活检组织中的20份（49%）检测到了HPV衣壳蛋白。所有宫颈DNA样本均与HPV 6/11探针杂交，两份还与HPV 16/18探针反应。
通过锥切术先前治疗过CIN III的女性中，85%可检测到宫颈或外阴的潜伏性HPV感染。
