Biomedical Engineering Program, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan.
Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
Drug Deliv Transl Res. 2017 Oct;7(5):609-616. doi: 10.1007/s13346-017-0382-7.
Needle-free jet injections constitute a crucial method for drug delivery. A novel liquid drug delivery system has been proposed recently, in which pressure atomizes liquid before delivering that atomized liquid to the patient's body; however, the mechanism and efficiency of the system are unclear. This study explored the shot delivery pressure, penetration depth, and cumulative amount of permeation of this system. This system was used to deliver 0.5% (w/v) methylene blue to agarose phantoms at various shot delivery pressures. Shots of methylene blue were also delivered to porcine skin samples at different shot delivery frequencies for light microscopy evaluation. A commercial microneedle array was used for comparing the effectiveness of the skin penetration depths. The array was gently stamped against porcine skin; methylene blue was subsequently applied to the area for different time points, followed by microscopic observations. In vitro skin penetration was tested using static Franz diffusion cells over 8 h. Finally, the feasibility of the system's clinical application was evaluated by analyzing the local analgesic effect in a heat nociceptive animal model. The penetration depths created using 100 shots at 100 psi were similar to those created using the commercial microneedle array for 2 h. Thermal stimulation responses showed that 15 min after diclofenac sodium was delivered by the system, heat nociception was significantly attenuated for 60 min. Our study presents a novel delivery system that may be useful for future clinical applications.
无针喷射注射是药物输送的重要方法。最近提出了一种新型的液体药物输送系统，该系统在将雾化的液体输送到患者体内之前使用压力将液体雾化；然而，该系统的机制和效率尚不清楚。本研究探讨了该系统的注射输送压力、穿透深度和累积渗透量。该系统用于以不同的注射输送压力将 0.5%（w/v）亚甲蓝输送到琼脂糖模型中。亚甲蓝还以不同的注射输送频率输送到猪皮样本，用于光镜评估。商业微针阵列用于比较皮肤穿透深度的效果。将微针阵列轻轻压在猪皮上；随后将亚甲蓝施加到该区域不同的时间点，然后进行显微镜观察。在体外皮肤渗透测试中，使用静态 Franz 扩散细胞进行 8 小时测试。最后，通过分析热伤害动物模型中的局部镇痛效果来评估该系统临床应用的可行性。以 100psi 进行 100 次注射所产生的穿透深度与商用微针阵列在 2 小时内产生的穿透深度相似。热刺激反应表明，在该系统输送双氯芬酸钠 15 分钟后，热伤害的感知明显减弱了 60 分钟。本研究提出了一种新的输送系统，可能对未来的临床应用有用。
