Nilsen Bo W, Jensen Einar, Örtengren Ulf, Michelsen Vibeke B
Department of Clinical Dentistry, UiT - The Arctic University of Norway, Tromsø, Norway.
Department of Pharmacy, UiT - The Arctic University of Norway, Tromsø, Norway.
Eur J Oral Sci. 2017 Jun;125(3):183-194. doi: 10.1111/eos.12347. Epub 2017 Apr 26.
The purpose of this study was to elucidate the organic composition and eluates of three resin-based pulp-capping materials in relation to their indications and safety data sheets. Uncured samples of Theracal LC, Ultra-Blend Plus, and Calcimol LC were investigated using gas chromatography-mass spectrometry (GC-MS) and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Identification/quantification of 7-d leachables of cured samples was performed using GC-MS for 2-hydroxyethyl methacrylate (HEMA), 2-(dimethylamino)ethyl methacrylate (DMAEMA), camphorquinone (CQ), ethylene glycol dimethacrylate (EGDMA), ethyl-4-(dimethylamino)benzoate (DMABEE), and triethylene glycol dimethacrylate (TEGDMA). A similar organic composition was found for Ultra-Blend and Calcimol; however, only Ultra-Blend is indicated for direct pulp-capping. In contrast to the other materials analysed, Theracal contained substances of high molecular weight. The safety data sheets of all materials were incomplete. We detected HEMA, CQ, and TEGDMA in eluates from Ultra-Blend and Calcimol, and it was considered that HEMA might have originated from decomposition of diurethane dimethacrylate (UDMA) in the GC-injector. For Theracal, additives associated with light curing (DMABEE and CQ) were detected in higher amounts (4.11 and 19.95 μg mm ) than in the other materials. Pores were quantified in all samples by micro-computed tomography (micro-CT) analysis, which could influence leaching. The organic substances in the investigated materials might affect their clinical suitability as capping agents, especially for direct capping procedures.
本研究的目的是阐明三种树脂基盖髓材料的有机成分和洗脱液，并与它们的适应症和安全数据表相关联。使用气相色谱-质谱联用仪（GC-MS）和超高效液相色谱-质谱联用仪（UPLC-MS）对Theracal LC、Ultra-Blend Plus和Calcimol LC的未固化样品进行了研究。使用GC-MS对固化样品的7天可浸出物进行鉴定/定量，分析甲基丙烯酸2-羟乙酯（HEMA）、甲基丙烯酸2-（二甲氨基）乙酯（DMAEMA）、樟脑醌（CQ）、乙二醇二甲基丙烯酸酯（EGDMA）、4-（二甲氨基）苯甲酸乙酯（DMABEE）和三乙二醇二甲基丙烯酸酯（TEGDMA）。Ultra-Blend和Calcimol的有机成分相似；然而，只有Ultra-Blend被指定用于直接盖髓。与其他分析材料相比，Theracal含有高分子量物质。所有材料的安全数据表都不完整。我们在Ultra-Blend和Calcimol的洗脱液中检测到了HEMA、CQ和TEGDMA，并且认为HEMA可能源自GC进样器中二甲基丙烯酸二聚氨酯（UDMA）的分解。对于Theracal，与光固化相关的添加剂（DMABEE和CQ）的检测量（分别为4.11和19.95μg/mm）高于其他材料。通过微计算机断层扫描（micro-CT）分析对所有样品中的孔隙进行了定量，孔隙可能会影响浸出。所研究材料中的有机物质可能会影响它们作为盖髓剂的临床适用性，特别是对于直接盖髓程序。
