Kallianta Meletia, Pappa Eftychia, Vastardis Heleni, Rahiotis Christos
Department of Operative Dentistry, School of Dentistry, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Department of Orthodontics, School of Dentistry, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Biomedicines. 2023 Jan 19;11(2):286. doi: 10.3390/biomedicines11020286.
Mass Spectrometry (MS) is one of the fastest-developing methods in analytical instrumentation. As a highly sensitive, universal detector, it can identify known and unknown compounds, which can indeed be found in a minimal concentration. This review aims to highlight the significant milestones in MS applications in dentistry during recent decades. MS can be applied in three different fields of dentistry: (1) in research of dental materials and chemical agents, (2) in laboratory analysis of biospecimens, and (3) as a real-time diagnostic tool in service of oral surgery and pathology. MS applications on materials and agents may focus on numerous aspects, such as their clinical behavior, possible toxicity, or antimicrobial properties. MS is also a valuable, non-invasive tool for biomarkers' detection in saliva and has found great application in -omics technologies as it achieves efficient structure-finding in metabolites. As metabolites are located beyond the central dogma, this technique can provide a complete understanding of cellular functions. Thus, it is possible to determine the biological profile in normal and pathological conditions, detect various oral or systematic diseases and conditions, and predict their course. Lastly, some promising advances concerning the surgical approach to potentially oral malignant or malignant disorders exist. This breakthrough method provides a comprehensive approach to dental materials research and biomarker discovery in dental and craniofacial tissues. The current availability of various 'OMIC' approaches paves the way for individualized dentistry and provides suggestions for clinical applications in the point-of-care hubs.
质谱分析法（MS）是分析仪器中发展最快的方法之一。作为一种高灵敏度的通用检测器，它能够识别已知和未知化合物，即使这些化合物的浓度极低也能被检测到。本综述旨在突出近几十年来质谱分析法在牙科应用中的重要里程碑。质谱分析法可应用于牙科的三个不同领域：（1）牙科材料和化学试剂的研究；（2）生物样本的实验室分析；（3）作为口腔外科和病理学的实时诊断工具。质谱分析法在材料和试剂方面的应用可能集中在许多方面，例如它们的临床行为、潜在毒性或抗菌特性。质谱分析法也是检测唾液中生物标志物的一种有价值的非侵入性工具，并且由于它能够在代谢物中高效地进行结构鉴定，因此在组学技术中得到了广泛应用。由于代谢物位于中心法则之外，这项技术能够全面了解细胞功能。因此，有可能确定正常和病理状态下的生物学特征，检测各种口腔或全身性疾病及状况，并预测其发展进程。最后，在针对潜在口腔恶性或恶性疾病的手术方法方面存在一些有前景的进展。这种突破性方法为牙科材料研究以及牙科和颅面组织中的生物标志物发现提供了一种全面的方法。当前各种“组学”方法的可用性为个性化牙科铺平了道路，并为即时护理中心的临床应用提供了建议。
