Nilsen Bo W, Jensen Einar, Örtengren Ulf, Bang Berit, Michelsen Vibeke B
Department of Clinical Dentistry, UiT - The Arctic University of Norway, Tromsø, Norway.
Department of Pharmacy, UiT - The Arctic University of Norway, Tromsø, Norway.
Eur J Oral Sci. 2019 Oct;127(5):425-434. doi: 10.1111/eos.12646. Epub 2019 Jul 16.
Dental composite dust has been shown to act as a vehicle for methacrylates in vivo/in vitro. The objective of this study was to assess airborne exposure of dental personnel to gaseous and particle-associated organic constituents from resin-based dental materials in a simulated clinic. Sampling of total aerosol fractions and gaseous substances was performed by dental students carrying particle filters and gas sorbents attached to a personal pump during preclinical restorative procedures in phantom models (n = 13). Water from the phantoms was sampled. Organic substances were extracted from the sampled water, particle filters, and gas sorbents. Qualitative and quantitative analyses were performed by gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS). The methacrylates 2-hydroxyethyl methacrylate (HEMA) and triethylene glycol dimethacrylate (TEGDMA) and the additives camphorquinone (CQ), butylated hydroxytoluene (BHT), and ethyl 4-(dimethylamino)benzoate (DMABEE), were quantified in the gas and particle fractions sampled. A positive-control experiment was conducted. No methacrylates were detected in the gas or particle fractions sampled, whereas strong signals for methacrylates were detected in the positive controls, matching the analysis of the uncured material. In addition, TEGDMA and DMABEE were quantified in the sampled water. Airborne exposure to constituents in resin-based dental materials was below the detection limit. However, the extent of exposure is probably dependent on the procedure, preventive measures, and type of materials used.
牙科复合树脂粉尘已被证明在体内/体外可作为甲基丙烯酸酯的载体。本研究的目的是在模拟诊所中评估牙科工作人员暴露于树脂基牙科材料中气态和颗粒相关有机成分的情况。在 phantom 模型（n = 13）的临床前修复程序中，牙科学生使用连接到个人泵上的颗粒过滤器和气体吸附剂对总气溶胶组分和气态物质进行采样。对 phantom 中的水进行采样。从采样的水、颗粒过滤器和气体吸附剂中提取有机物质。通过气相色谱 - 质谱联用（GC - MS）和超高效液相色谱 - 质谱联用（UHPLC - MS）进行定性和定量分析。对采样的气体和颗粒组分中的甲基丙烯酸酯 2 - 羟乙基甲基丙烯酸酯（HEMA）和三乙二醇二甲基丙烯酸酯（TEGDMA）以及添加剂樟脑醌（CQ）、丁基化羟基甲苯（BHT）和 4 - （二甲基氨基）苯甲酸乙酯（DMABEE）进行了定量分析。进行了阳性对照实验。在采样的气体或颗粒组分中未检测到甲基丙烯酸酯，而在阳性对照中检测到了甲基丙烯酸酯的强信号，与未固化材料的分析结果相符。此外，在采样的水中对 TEGDMA 和 DMABEE 进行了定量分析。树脂基牙科材料中成分的空气传播暴露低于检测限。然而，暴露程度可能取决于操作程序、预防措施和所用材料的类型。
