非人灵长类动物中副结核分枝杆菌亚种的检测。
Detection of Mycobacterium avium subsp. paratuberculosis in non-human primates.
作者信息
Fechner Kim, Mätz-Rensing Kerstin, Lampe Karen, Kaup Franz-Josef, Czerny Claus-Peter, Schäfer Jenny
机构信息
Division of Microbiology and Animal Hygiene, Department of Animal Sciences, Faculty of Agricultural Sciences, University of Goettingen, Göttingen, Germany.
Pathology Unit, German Primate Center (DPZ), Göttingen, Germany.
出版信息
J Med Primatol. 2017 Oct;46(5):211-217. doi: 10.1111/jmp.12270. Epub 2017 Apr 26.
BACKGROUND
Due to a sporadic occurrence of Mycobacterium avium subsp. paratuberculosis (MAP) in non-human primates (NHP), the susceptibility of different NHP to MAP should be investigated.
METHODS
Fecal and tissue samples (ileum, ileocecal lymph node, bone marrow) of 20 animals (seven species) were analyzed by IS900-based PCRs and sequenced. Samples of MAP PCR positive NHP were further cultivated.
RESULTS
MAP DNA was detectable in two animals; the ileum of a cottontop tamarin and the bone marrow of a common marmoset. Cultivation of MAP failed. Sequence analysis revealed 100% homology to the MAP-K10 sequence. Pathohistological examinations offered no direct correlation to a MAP infection.
CONCLUSIONS
MAP was detected for the first time in a common marmoset. But as both NHP suffered from other diseases, an asymptomatic infection with MAP was assumed. The detection of MAP in the bone marrow might play a role in establishing latent paratuberculosis, as known from tuberculosis.
背景
由于非人类灵长类动物（NHP）中偶发鸟分枝杆菌副结核亚种（MAP），应研究不同NHP对MAP的易感性。
方法
对20只动物（7个物种）的粪便和组织样本（回肠、回盲部淋巴结、骨髓）进行基于IS900的聚合酶链反应（PCR）分析并测序。对MAP PCR阳性的NHP样本进一步培养。
结果
在两只动物中检测到MAP DNA；一只棉顶狨的回肠和一只普通狨猴的骨髓。MAP培养失败。序列分析显示与MAP-K10序列100%同源。病理组织学检查未发现与MAP感染有直接关联。
结论
首次在普通狨猴中检测到MAP。但由于这两只NHP都患有其他疾病，推测为MAP无症状感染。骨髓中检测到MAP可能在建立潜伏性副结核中起作用，这与结核病情况类似。
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