Hang Pham Le B, Yoo Yie-Ri, Park Sun Hwa, Back Sang A, Kim Sung Won, Bjørge Isabel, Mano João, Jang Jun-Hyeog
Department of Biochemistry, Inha University School of Medicine, Incheon, 22212, Korea.
Department of Biomedical Science, The Catholic University of Korea, College of Medicine, Seoul Korea.
J Biomed Mater Res A. 2017 Aug;105(8):2291-2298. doi: 10.1002/jbm.a.36095. Epub 2017 May 17.
Many extracellular matrix proteins have positive influences on the adhesion, proliferation, and differentiation of stem cells into specific cell linages. Fibulin-1 (FBLN1), a member of a growing family of extracellular glycoproteins, contributes to the structure of the extracellular matrix. Here, we investigated the effect of FBLN1 on the ability of human nasal inferior turbinate-derived mesenchymal stem cells (hTMSCs) to undergo osteogenic differentiation. After we generated recombinant FBLN1, the characteristics of FBLN1-treated hTMSCs were evaluated using MTT assay, ALP and mineralization activities, and quantitative real-time PCR. FBLN1 significantly enhanced the adhesion activity (p < 0.001) and proliferation of hTMSCs (p < 0.05). The ALP and mineralization activities of cells were dramatically increased (p < 0.01) after 9 and 12 days of FBLN1 treatment, respectively. This indicated the ability of FBLN1 to induce hTMSCs to differentiate into osteoblasts. Furthermore, increasing the mRNA levels of osteogenic marker genes, such as a transcriptional coactivator with a PDZ-binding motif (TAZ), alkaline phosphatase (ALP), collagen type I (Col I), and osteocalcin (OCN), improved bone repair and regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2291-2298, 2017.
许多细胞外基质蛋白对干细胞黏附、增殖以及向特定细胞谱系分化具有积极影响。纤连蛋白-1（FBLN1）是细胞外糖蛋白家族中不断增加的一员，对细胞外基质结构有重要作用。在此，我们研究了FBLN1对人下鼻甲来源间充质干细胞（hTMSCs）成骨分化能力的影响。在制备重组FBLN1后，通过MTT法、碱性磷酸酶（ALP）和矿化活性以及定量实时PCR对经FBLN1处理的hTMSCs的特性进行评估。FBLN1显著增强了hTMSCs的黏附活性（p < 0.001）和增殖能力（p < 0.05）。在FBLN1处理9天和12天后，细胞的ALP和矿化活性分别显著增加（p < 0.01）。这表明FBLN1具有诱导hTMSCs分化为成骨细胞的能力。此外，增加成骨标记基因的mRNA水平，如具有PDZ结合基序的转录共激活因子（TAZ）、碱性磷酸酶（ALP）、I型胶原蛋白（Col I）和骨钙素（OCN），可促进骨修复和再生。© 2017威利期刊公司。《生物医学材料研究杂志》A部分：第105A卷：2291 - 2298页，2017年。
