注射到非洲爪蟾卵母细胞中的DNA模板之间的竞争。

Competition between DNA templates injected into Xenopus laevis oocytes.

作者信息

Mancebo M J, Etkin L D

机构信息

Department of Molecular Genetics, University of Texas Systems Cancer Center, M.D. Anderson Hospital and Tumor Institute, Houston 77030.

出版信息

Exp Cell Res. 1988 Oct;178(2):469-78. doi: 10.1016/0014-4827(88)90415-6.

DOI:10.1016/0014-4827(88)90415-6

PMID:2844564

Abstract

We have examined the competition between microinjected DNA templates for endogenous Xenopus oocyte transcription factors. The results suggest that (a) coinjected templates compete with one another for transcription factors, (b) the competitive ability of a template is not necessarily a function of the rate of transcription of the gene, (c) competition is due to the presence of specific DNA sequences, and (d) preincubation of the test gene affects competition.

摘要

我们研究了显微注射的DNA模板对内源性非洲爪蟾卵母细胞转录因子的竞争情况。结果表明：（a）共注射的模板相互竞争转录因子；（b）模板的竞争能力不一定是基因转录速率的函数；（c）竞争是由于特定DNA序列的存在；（d）测试基因的预孵育会影响竞争。