Harland R M, Weintraub H, McKnight S L
Nature. 1983 Mar 3;302(5903):38-43. doi: 10.1038/302038a0.
We have assayed the transcription of the herpes simplex virus thymidine kinase gene on circular and linear plasmids after injection into Xenopus oocytes. The circular plasmids appear to be transcribed at least 500 times more efficiently than the linear plasmids. Moreover, when the circular templates are allowed to form functioning transcription complexes, and are then linearized in situ, the synthesis of accurate transcripts is substantially reduced.
我们检测了注射到非洲爪蟾卵母细胞中的环状和线性质粒上单纯疱疹病毒胸苷激酶基因的转录情况。环状质粒的转录效率似乎比线性质粒至少高500倍。此外，当环状模板形成功能性转录复合物后，再在原位将其线性化，准确转录本的合成会大幅减少。
