圆线虫感染对西部低地大猩猩和东部黑猩猩粪便中疟原虫属检测无影响。

No impact of strongylid infections on the detection of Plasmodium spp. in faeces of western lowland gorillas and eastern chimpanzees.

作者信息

Mapua Mwanahamisi I, Pafčo Barbora, Burgunder Jade, Profousová-Pšenková Ilona, Todd Angelique, Hashimoto Chie, Qablan Moneeb A, Modrý David, Petrželková Klára J

机构信息

Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1946/1, 612 42, Brno, Czech Republic.

Faculty of Science, Masaryk University, Kotlářská 267/2, 611 37, Brno, Czech Republic.

出版信息

Malar J. 2017 Apr 26;16(1):175. doi: 10.1186/s12936-017-1822-z.

DOI:10.1186/s12936-017-1822-z

PMID:28446233

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5406944/

Abstract

BACKGROUND

Although a high genetic diversity of Plasmodium spp. circulating in great apes has been revealed recently due to non-invasive methods enabling detection in faecal samples, little is known about the actual mechanisms underlying the presence of Plasmodium DNA in faeces. Great apes are commonly infected by strongylid nematodes, including hookworms, which cause intestinal bleeding. The impact of strongylid infections on the detection of Plasmodium DNA in faeces was assessed in wild, western, lowland gorillas from Dzanga Sangha Protected Areas, Central African Republic and eastern chimpanzees from Kalinzu Forest Reserve, Uganda.

METHODS

Fifty-one faecal samples from 22 habituated gorillas and 74 samples from 15 habituated chimpanzees were analysed using Cytochrome-b PCR assay and coprological methods.

RESULTS

Overall, 26.4% of the analysed samples were positive for both Plasmodium spp. and strongylids. However, the results showed no significant impact of intensity of infections of strongylids on detection of Plasmodium DNA in gorilla and chimpanzee faeces.

CONCLUSION

Bleeding caused by strongylid nematode Necator spp. cannot explain the presence of Plasmodium DNA in ape faeces.

摘要

背景

尽管由于非侵入性方法能够在粪便样本中检测到，最近在大猩猩中循环的疟原虫属的高遗传多样性已被揭示，但对于粪便中疟原虫DNA存在的实际潜在机制知之甚少。大猩猩通常感染包括钩虫在内的圆线虫，这些线虫会导致肠道出血。在来自中非共和国Dzanga Sangha保护区的野生西部低地大猩猩和来自乌干达Kalinzu森林保护区的东部黑猩猩中，评估了圆线虫感染对粪便中疟原虫DNA检测的影响。