组织样本的蛋白质组学分析揭示甲状旁腺增生相对于腺瘤中线粒体蛋白水平的变化。
Proteomics Analysis of Tissue Samples Reveals Changes in Mitochondrial Protein Levels in Parathyroid Hyperplasia over Adenoma.
作者信息
Akpinar Gurler, Kasap Murat, Canturk Nuh Zafer, Zulfigarova Mehin, Islek Eylül Ece, Guler Sertac Ata, Simsek Turgay, Canturk Zeynep
机构信息
Department of Medical Biology and Genetics, School of Medicine, Kocaeli University, Kocaeli, Turkey
DEKART Proteomics Laboratory, Kocaeli University, Kocaeli, Turkey.
出版信息
Cancer Genomics Proteomics. 2017 May-Jun;14(3):197-211. doi: 10.21873/cgp.20031.
BACKGROUND/AIM: To unveil the pathophysiology of primary hyperparathyroidism, molecular details of parathyroid hyperplasia and adenoma have to be revealed. Such details will provide the tools necessary for differentiation of these two look-alike diseases. Therefore, in the present study, a comparative proteomic study using postoperative tissue samples from the parathyroid adenoma and parathyroid hyperplasia patients was performed.
MATERIALS AND METHODS
Protein extracts were prepared from tissue samples (n=8 per group). Protein pools were created for each group and subjected to DIGE and conventional 2DE. Following image analysis, spots representing the differentially regulated proteins were excised from the and used for identification via MALDI-TOF/TOF analysis.
RESULTS
The identities of 40 differentially-expressed proteins were revealed. Fourteen of these proteins were over-expressed in the hyperplasia while 26 of them were over-expressed in the adenoma.
CONCLUSION
Most proteins found to be over-expressed in the hyperplasia samples were mitochondrial, underlying the importance of the mitochondrial activity as a potential biomarker for differentiation of parathyroid hyperplasia from adenoma.
背景/目的：为揭示原发性甲状旁腺功能亢进症的病理生理学，必须了解甲状旁腺增生和腺瘤的分子细节。这些细节将为区分这两种相似疾病提供必要的工具。因此，在本研究中，我们对甲状旁腺腺瘤和甲状旁腺增生患者的术后组织样本进行了比较蛋白质组学研究。
材料与方法
从组织样本中制备蛋白质提取物（每组n = 8）。为每组创建蛋白质池，并进行差异凝胶电泳（DIGE）和传统的二维电泳（2DE）。图像分析后，从凝胶中切下代表差异调节蛋白的斑点，并通过基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF/TOF）分析进行鉴定。
结果
揭示了40种差异表达蛋白的身份。其中14种蛋白在增生组织中过度表达，26种在腺瘤中过度表达。
结论
在增生样本中发现过度表达的大多数蛋白是线粒体蛋白，这突出了线粒体活性作为区分甲状旁腺增生和腺瘤的潜在生物标志物的重要性。
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