Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.
Department of Rheumatology, GIGA Research, Centre Hospitalier Universitaire (CHU) de Liège, University of Liège, Liège, Belgium.
Cancer Genomics Proteomics. 2021 Nov-Dec;18(6):781-796. doi: 10.21873/cgp.20297.
BACKGROUND/AIM: The lack of specific parathyroid carcinoma (PC) biomarkers in clinical practice points out the importance of analyzing the proteomic signature of this cancer. We performed a comparative proteomic analysis of PC and parathyroid adenoma (PA) co-existing in the same patient.
PC and PA were taken from a 63-year-old patient. Using two-dimensional differential gel electrophoresis (2D-DIGE) coupled to mass spectrometry we examined the differences between PC and PA proteins. For validation, additional PC and PA samples were obtained from 10 patients. Western blot analysis was used to validate the difference of expression observed with 2D-DIGE analysis. Bioinfomatic analysis was performed using QIAGEN's Ingenuity Pathways Analysis (IPA) to determine the predominant canonical pathways and interaction networks involved.
Thirty-three differentially expressed proteins were identified in PC compared to PA. Among these, ubiquitin C-terminal hydrolase-L1 (UCH-L1) was highly overexpressed in PC. The result was confirmed by Western Blot analysis in additional PC samples.
Our comparative proteomic analysis of co-existing neoplasms allowed detecting specific and peculiar differences between PC and PA overcoming population biological variability.
背景/目的：临床上缺乏特定的甲状旁腺癌（PC）生物标志物，这凸显了分析这种癌症蛋白质组特征的重要性。我们对同一患者中并存的 PC 和甲状旁腺腺瘤（PA）进行了比较蛋白质组学分析。
从一名 63 岁患者身上采集 PC 和 PA。我们使用二维差异凝胶电泳（2D-DIGE）结合质谱法检查了 PC 和 PA 蛋白之间的差异。为了验证，从另外 10 名患者中获得了额外的 PC 和 PA 样本。使用 Western blot 分析来验证 2D-DIGE 分析观察到的表达差异。使用 QIAGEN 的 Ingenuity Pathways Analysis（IPA）进行生物信息学分析，以确定涉及的主要规范途径和相互作用网络。
与 PA 相比，PC 中鉴定出 33 种差异表达蛋白。其中，泛素羧基末端水解酶-L1（UCH-L1）在 PC 中高度过表达。这一结果在额外的 PC 样本中通过 Western blot 分析得到了证实。
我们对并存肿瘤的比较蛋白质组学分析能够检测到 PC 和 PA 之间的特定且独特的差异，克服了群体生物学变异性。
