Samad Abdul F A, Sajad Muhammad, Nazaruddin Nazaruddin, Fauzi Izzat A, Murad Abdul M A, Zainal Zamri, Ismail Ismanizan
School of Biosciences and Biotechnology, Faculty of Science and Technology, National University of Malaysia, SelangorMalaysia.
Department of Plant Breeding and Genetics, University College of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, PunjabPakistan.
Front Plant Sci. 2017 Apr 12;8:565. doi: 10.3389/fpls.2017.00565. eCollection 2017.
Recent achievements in plant microRNA (miRNA), a large class of small and non-coding RNAs, are very exciting. A wide array of techniques involving forward genetic, molecular cloning, bioinformatic analysis, and the latest technology, deep sequencing have greatly advanced miRNA discovery. A tiny miRNA sequence has the ability to target single/multiple mRNA targets. Most of the miRNA targets are transcription factors (TFs) which have paramount importance in regulating the plant growth and development. Various families of TFs, which have regulated a range of regulatory networks, may assist plants to grow under normal and stress environmental conditions. This present review focuses on the regulatory relationships between miRNAs and different families of TFs like; NF-Y, MYB, AP2, TCP, WRKY, NAC, GRF, and SPL. For instance NF-Y play important role during drought tolerance and flower development, MYB are involved in signal transduction and biosynthesis of secondary metabolites, AP2 regulate the floral development and nodule formation, TCP direct leaf development and growth hormones signaling. WRKY have known roles in multiple stress tolerances, NAC regulate lateral root formation, GRF are involved in root growth, flower, and seed development, and SPL regulate plant transition from juvenile to adult. We also studied the relation between miRNAs and TFs by consolidating the research findings from different plant species which will help plant scientists in understanding the mechanism of action and interaction between these regulators in the plant growth and development under normal and stress environmental conditions.
植物微小RNA（miRNA）是一大类小分子非编码RNA，其近期的研究成果十分令人振奋。一系列技术，包括正向遗传学、分子克隆、生物信息学分析以及最新的深度测序技术，极大地推动了miRNA的发现。一个微小的miRNA序列能够靶向单个/多个mRNA靶标。大多数miRNA靶标是转录因子（TFs），它们在调节植物生长发育方面至关重要。各种转录因子家族调控着一系列调控网络，可帮助植物在正常和胁迫环境条件下生长。本综述重点关注miRNA与不同转录因子家族之间的调控关系，如NF-Y、MYB、AP2、TCP、WRKY、NAC、GRF和SPL。例如，NF-Y在耐旱性和花发育过程中发挥重要作用，MYB参与次生代谢物的信号转导和生物合成，AP2调节花发育和根瘤形成，TCP指导叶片发育和生长激素信号传导。WRKY在多种胁迫耐受性中发挥已知作用，NAC调节侧根形成，GRF参与根生长、花和种子发育，SPL调节植物从幼年到成年的转变。我们还通过整合来自不同植物物种的研究结果，研究了miRNA与转录因子之间的关系，这将有助于植物科学家理解这些调控因子在正常和胁迫环境条件下植物生长发育中的作用机制和相互作用。
