Department of Neurology, University Hospital Essen, Essen, Germany.
Institute of Molecular and Cell Biology, A*STAR, 61 Biopolis Drive, Singapore, Singapore.
Cell Mol Neurobiol. 2018 Mar;38(2):487-496. doi: 10.1007/s10571-017-0494-0. Epub 2017 Apr 26.
The protein component of the myelin layer is essential for all aspects of peripheral nerves, and its deficiency can lead to structural and functional impairment. The presence of peripheral myelin protein 2 (P2, PMP2, FABP8, M-FABP) in Schwann cells has been known for decades and shown recently to be involved in the lipid homeostasis in the peripheral neural system. However, its precise role during de- and remyelination has yet to be elucidated. To this end, we assessed remyelination after sciatic nerve crush injury in vivo, and in an experimental de/remyelination ex vivo myelinating culture model in P2-deficient (P2 ) and wild-type (WT) animals. In vivo, the nerve crush paradigm revealed temporal structural and functional changes in P2 mice as compared to WT animals. Concomitantly, P2 DRG cultures demonstrated the presence of shorter internodes and enlarged nodes after ex vivo de/remyelination. Together, these data indicate that P2 may play a role in remyelination of the injured peripheral nervous system, presumably by affecting the nodal and internodal configuration.
髓鞘层的蛋白质成分对周围神经的各个方面都是必不可少的，其缺乏可导致结构和功能损伤。几十年来，人们一直知道施万细胞中存在外周髓鞘蛋白 2（P2、PMP2、FABP8、M-FABP），最近的研究表明它参与了周围神经系统的脂质动态平衡。然而，其在脱髓鞘和髓鞘再生过程中的确切作用仍有待阐明。为此，我们评估了体内坐骨神经挤压损伤后的髓鞘再生，以及在 P2 缺陷（P2-/-）和野生型（WT）动物的实验性脱髓鞘/再髓鞘体外髓鞘形成培养模型中的髓鞘再生。在体内，与 WT 动物相比，神经挤压模型显示 P2-/- 小鼠在时间上存在结构和功能变化。同时，P2-/-DRG 培养物在体外脱髓鞘/再髓鞘后显示出较短的节间和增大的节点。总之，这些数据表明 P2 可能通过影响节点和节间的结构来发挥作用，从而在损伤的周围神经系统的髓鞘再生中发挥作用。
