Chahdoura Hassiba, El Bok Safia, Refifa Taoufik, Adouni Khaoula, Khemiss Fethia, Mosbah Habib, Ben-Attia Mossadok, Flamini Guido, Achour Lotfi
Laboratoire de Recherche "Bioressourses, Biologie Intégrative & Valorisation", Institut Supérieur de Biotechnologie de Monastir, Université de Monastir, Monastir, Tunisia.
Laboratoire de Biodiversité, Biotechnologies et Changements Climatiques, Faculté' des Sciences de Tunis, Université Tunis-El Manar, Tunis, Tunisia.
J Pharm Pharmacol. 2017 Aug;69(8):1056-1063. doi: 10.1111/jphp.12734. Epub 2017 Apr 26.
The aim of this study was to evaluate the analgesic and the anti-inflammatory activity of Opuntia microdasys at post flowering stage, F3 (OMF3) in rat and, in other hand, its antigenotoxic effects by the Allium cepa test.
OMF3 extracts were screened for activity of analgesic and anti-inflammatory using, respectively, the acetic acid writhing test in mice and the carrageenan-induced paw oedema assay in rats. The antigenotoxic has been evaluated by A. cepa test.
OMF3 extracts showed a higher analgesic and anti-inflammatory activity at 100 mg/kg (72.03% and 70.11%) as determined by the tests of acetic acid-induced writhing and carrageenan-induced oedema, respectively. Furthermore, the OMF3 aqueous extracts have a preventive antimutagenic potential, at lower concentration (EC ≈ 60 μg/ml), against H O -mediated DNA damage in A. cepa root meristem cells with an efficient restoration of the mitotic index in comparison with controls.
Based on this study, the flower of O. microdasys at post flowering stage may be use as an analgesic, anti-inflammatory and antimutagenic agents.
本研究旨在评估仙人掌科植物小花刺梨开花后期F3（OMF3）在大鼠体内的镇痛和抗炎活性，以及通过洋葱根尖试验评估其抗遗传毒性作用。
分别采用小鼠醋酸扭体试验和大鼠角叉菜胶诱导足跖肿胀试验，对OMF3提取物的镇痛和抗炎活性进行筛选。通过洋葱根尖试验评估其抗遗传毒性。
通过醋酸诱导扭体试验和角叉菜胶诱导水肿试验测定，OMF3提取物在100mg/kg时分别表现出较高的镇痛（72.03%）和抗炎活性（70.11%）。此外，OMF3水提取物在较低浓度（EC≈60μg/ml）下，对过氧化氢介导的洋葱根尖分生组织细胞DNA损伤具有预防抗诱变潜力，与对照组相比，有丝分裂指数能有效恢复。
基于本研究，小花刺梨开花后期的花朵可作为镇痛、抗炎和抗诱变剂使用。
