Laboratory of Bioresources: Integrative Biology and Exploiting, Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Taher Hadded BP 74, Monastir 5000, Tunisia.
Unité de Recherche "Génomique, Biotechnologie et Stratégies Antivirales", Institut Supérieur de Biotechnologie, Université de Monastir, BP74, Avenue Tahar Hadded, Monastir 5000, Tunisia.
Molecules. 2023 Jun 29;28(13):5096. doi: 10.3390/molecules28135096.
Wild (VAC) is a Mediterranean plant that is rich in bioactive metabolites. This study aimed to validate, for the first time, the beneficial use of VAC fruits and fruit decoctions (VFDs) through in vitro and in vivo trials. Forty-one volatile components were detected in VAC fruits, with 1,8-cineole (30.3%) comprising the majority. The antioxidant activity of VFD was measured by using different in vitro methods (EC of 0.16 mg/mL by β-carotene bleaching inhibition assay) and by measuring the DNA protection power. Using the disc diffusion assay, the antimicrobial activity of VFD was evaluated, and it exhibited a noticeable anticandidal activity. VFD did not cause any toxicity or mortality in rats treated with doses > 200 mg/kg. Using the acetic acid writhing test, the antinociceptive activity of VFD was measured. Our results showed that VFD at 200 mg/kg exhibited a higher analgesic activity (81.68%) than acetylsalicylic acid used as a positive control (74.35%). Its gastroprotective ability was assessed by HCl/ethanol-induced gastric lesions, which were remarkably inhibited (84.62%) by intraperitoneal administration of VFD. This work helps to validate the popular use of VAC to treat nociceptive, inflammatory, and gastric disorders and encourages researchers to further investigate the identification of pharmacological compounds from this species.
野生（VAC）是一种富含生物活性代谢物的地中海植物。本研究旨在通过体外和体内试验首次验证 VAC 果实和果实煎剂（VFD）的有益用途。在 VAC 果实中检测到 41 种挥发性成分，其中 1,8-桉叶油醇（30.3%）含量最多。VFD 的抗氧化活性通过使用不同的体外方法（β-胡萝卜素漂白抑制试验的 EC 为 0.16mg/mL）和测量 DNA 保护能力来测量。使用圆盘扩散试验评估了 VFD 的抗菌活性，结果表明它具有明显的抗真菌活性。VFD 在大鼠中的最大剂量 >200mg/kg 时没有引起任何毒性或死亡率。通过醋酸扭体试验测量了 VFD 的镇痛活性。结果表明，VFD 在 200mg/kg 时的镇痛活性（81.68%）高于作为阳性对照的乙酰水杨酸（74.35%）。通过盐酸/乙醇诱导的胃损伤评估了 VFD 的胃保护能力，VFD 腹腔给药可显著抑制胃损伤（84.62%）。这项工作有助于验证 VAC 用于治疗疼痛、炎症和胃部疾病的流行用途，并鼓励研究人员进一步研究从该物种中鉴定药理化合物。
