Burton W G, Nugent K D, Slattery T K, Summers B R, Snyder L R
Bionovus, Inc., Foster City, CA 94404.
J Chromatogr. 1988 Jun 29;443:363-79. doi: 10.1016/s0021-9673(00)94808-8.
In the process of developing a new analytical technology (the chromatophoresis process) which couples reversed-phase high-performance liquid chromatography (HPLC) to sodium dodecyl sulfate polyacrylamide gel electrophoresis in a real-time automated system, it was apparent that improvements in resolving power for the first-dimension (HPLC) separation were necessary. The present paper describes the optimization of the column for our initial work on reversed-phase HPLC separations. Polymeric (polystyrene) packings having particle diameters of 5 micron and pore diameters of 300 A were generally superior in terms of resolution, sample recovery and minimization of "ghosting". Optimum column dimensions were 50 x 1.0 mm I.D. for the flow-rates required in our system (10-100 microliter/min).
在开发一种新的分析技术（色谱电泳法）的过程中，该技术将反相高效液相色谱（HPLC）与十二烷基硫酸钠聚丙烯酰胺凝胶电泳在一个实时自动化系统中结合起来，很明显，提高一维（HPLC）分离的分辨率是必要的。本文描述了在我们最初的反相HPLC分离工作中对色谱柱的优化。粒径为5微米、孔径为300埃的聚合物（聚苯乙烯）填料在分辨率、样品回收率和“拖尾”最小化方面通常更具优势。对于我们系统所需的流速（10 - 100微升/分钟），最佳柱尺寸为内径50 x 1.0毫米。
