Ferreri N R, Zeiger R S, Spiegelberg H L
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
J Allergy Clin Immunol. 1988 Oct;82(4):556-67. doi: 10.1016/0091-6749(88)90965-7.
Purified peripheral blood monocytes isolated from patients with atopic dermatitis (AD) and from nonallergic normal donors were compared for their abilities to release leukotriene C4 (LTC4), leukotriene B4 (LTB4) and beta-glucuronidase in response to challenge with aggregated immunoglobulins or anti-immunoglobulins. The relationship between mediator release and the number of monocytes that formed rosettes with immunoglobulin-coated indicator cells was examined. Patients with AD had twice as many IgA- and three times as many IgE-rosetting monocytes as normal donors (48 +/- 12% versus 27 +/- 10% and 40 +/- 15% versus 14 +/- 3%, respectively), and yet the amounts of IgA- and IgE-induced LTC4 released were similar for both groups. This apparent discrepancy did not result from a decreased capacity for arachidonate metabolism via the C5-lipoxygenase pathway, since stimulation of monocytes from patients and normal donors with the calcium ionophore A23187 induced similar amounts of LTC4 and LTB4 release (LTC4, 3.0 +/- 1.7 versus 3.0 +/- 1.0 ng/10(6) cells; LTB4, 5.3 +/- 0.7 versus 5.2 +/- 0.5 ng/10(6) cells, respectively). In addition, aggregated IgG-induced LTC4 release by monocytes of both groups was similar, concomitant with an equivalent number of IgG-rosetting cells. Determination of cytophilically bound IgG and IgE by flow cytometry demonstrated that monocytes from atopic patients had more IgG bound than monocytes from normal donors. Similar amounts of IgE were detected on most monocytes from both groups, despite the higher serum IgE levels of patients. However, approximately 3% to 8% of monocytes from atopic but not normal donors stained brightly for IgE, suggesting that relatively large amounts of cytophilic IgE were bound to a small percentage of the patients' monocytes. Challenge of monocytes with anti-IgE or anti-IgG induced release of similar amounts of LTC4 for both groups, despite the presence of more cytophilic IgG on monocytes from atopic donors. These data indicate that monocytes from patients with AD release LTC4 and LTB4 in response to challenge with aggregated IgE or anti-IgE, as well as aggregated IgG, IgA, and anti-IgG. However, under our in vitro conditions, stimulation of patients' monocytes with aggregated IgA or IgE was not associated with increased mediator release, despite higher percentages of IgA- and IgE-rosetting cells compared to normal donors.
比较了从特应性皮炎（AD）患者和非过敏性正常供体中分离出的纯化外周血单核细胞在受到聚集免疫球蛋白或抗免疫球蛋白刺激时释放白三烯C4（LTC4）、白三烯B4（LTB4）和β-葡萄糖醛酸酶的能力。研究了介质释放与与免疫球蛋白包被指示细胞形成花环的单核细胞数量之间的关系。AD患者中与IgA形成花环的单核细胞数量是正常供体的两倍，与IgE形成花环的单核细胞数量是正常供体的三倍（分别为48±12%对27±10%和40±15%对14±3%），然而两组中IgA和IgE诱导释放的LTC4量相似。这种明显的差异并非由于通过C5-脂氧合酶途径的花生四烯酸代谢能力降低，因为用钙离子载体A23187刺激患者和正常供体的单核细胞诱导释放的LTC4和LTB4量相似（LTC4，3.0±1.7对3.0±1.0 ng/10⁶细胞；LTB4，5.3±0.7对5.2±0.5 ng/10⁶细胞）。此外，两组单核细胞由聚集IgG诱导的LTC4释放相似，同时IgG花环形成细胞数量相当。通过流式细胞术测定嗜细胞性结合的IgG和IgE表明，特应性患者的单核细胞比正常供体的单核细胞结合更多的IgG。尽管患者血清IgE水平较高，但两组大多数单核细胞上检测到的IgE量相似。然而，特应性患者而非正常供体中约3%至8%的单核细胞IgE染色明亮，表明相对大量的嗜细胞性IgE与患者一小部分单核细胞结合。尽管特应性供体的单核细胞上存在更多嗜细胞性IgG，但用抗IgE或抗IgG刺激两组单核细胞诱导释放的LTC4量相似。这些数据表明，AD患者的单核细胞在受到聚集IgE或抗IgE以及聚集IgG、IgA和抗IgG刺激时释放LTC4和LTB4。然而，在我们的体外条件下，尽管与正常供体相比，IgA和IgE花环形成细胞百分比更高，但用聚集IgA或IgE刺激患者的单核细胞并未导致介质释放增加。
