State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, China.
Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Department of Histology and Embryology, School of Medicine, Nankai University, Tianjin 300071, China.
Chemosphere. 2017 Aug;181:270-280. doi: 10.1016/j.chemosphere.2017.04.094. Epub 2017 Apr 21.
All of these days, residues of herbicides such as glyphosate are widely distributed in the environment. The ubiquitous use of glyphosate has drawn extensive attention to its toxicity as an organic pollutant. In this study, we employed larval zebrafish as an animal model to evaluate the effect of different concentrations of glyphosate on early development via morphological, biomechanics, behavioral and physiological analyses. Morphological results showed that an obvious delay occurred in the epiboly process and body length, eye and head area were reduced at concentrations higher than 10 mg/L. The expression of ntl (no tail) shortened and krox20 (also known as Egr2b, early growth response 2b) changed as the glyphosate concentration increased, but there was no change in the expression of shh (sonic hedgehog). In addition, biomechanical analysis of the elasticity of chorion indicated that treated embryos' surface tension was declined. Furthermore, a 48-h locomotion test revealed that embryonic exposure to glyphosate significantly elevated locomotor activities, which is probably attributed to motoneuronal damage. The decreased surface tension of chorion and the increased locomotive activities may contribute to the hatching rates after glyphosate treatment. Our study enriches the researches of evaluating glyphosate toxicity and probablely plays a warning role in herbicides used in farming.
这些天来，草甘膦等除草剂的残留广泛分布在环境中。草甘膦的广泛使用使其作为有机污染物的毒性引起了广泛关注。在这项研究中，我们使用幼体斑马鱼作为动物模型，通过形态学、生物力学、行为和生理学分析来评估不同浓度的草甘膦对早期发育的影响。形态学结果表明，在浓度高于 10mg/L 时，胚环过程明显延迟，体长、眼睛和头部区域缩小。ntl（无尾）的表达缩短，krox20（也称为 Egr2b，早期生长反应 2b）随着草甘膦浓度的增加而改变，但 shh（sonic hedgehog）的表达没有变化。此外，对卵壳弹性的生物力学分析表明，处理后的胚胎表面张力下降。此外，48 小时的运动测试表明，胚胎暴露于草甘膦会显著增加运动活动，这可能归因于运动神经元损伤。卵壳表面张力的降低和运动活性的增加可能导致草甘膦处理后的孵化率增加。我们的研究丰富了评估草甘膦毒性的研究，可能对农业中使用的除草剂起到警示作用。
