Feng Gang, Sun Yuhua
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
Front Neurosci. 2022 Sep 1;16:960149. doi: 10.3389/fnins.2022.960149. eCollection 2022.
The development of central nervous system (CNS) and enteric nervous system (ENS) is under precise and strict control in vertebrates. Whether and how the Polycomb repressive complex 1 (PRC1) is involved in it remain unclear. To investigate the role of PRC1 in the nervous system development, using CRISPR/Cas9 technology, we have generated mutant zebrafish lines for the gene which encodes Ring1b, the enzymatic component of the PRC1 complex. We show that loss of function leads to abnormal migration and differentiation of neural crest and neural precursor cells. mutant embryos exhibit aganglionosis, in which the hindgut is devoid of neurons. In particular, the formation of 5-HT serotonin neurons and myelinating glial cells is defective. Furthermore, ectopic expression of ENS marker genes is observed in forebrain of mutant embryos. These findings suggest that the gene plays an important role in the migration and differentiation of neural precursor cells, and its absence leads to abnormal development of ENS and CNS in zebrafish.
在脊椎动物中，中枢神经系统（CNS）和肠神经系统（ENS）的发育受到精确且严格的控制。多梳抑制复合物1（PRC1）是否以及如何参与其中仍不清楚。为了研究PRC1在神经系统发育中的作用，我们利用CRISPR/Cas9技术构建了编码PRC1复合物酶组分Ring1b的基因的突变斑马鱼品系。我们发现功能缺失会导致神经嵴和神经前体细胞的迁移和分化异常。突变胚胎表现出神经节缺乏症，其中后肠没有神经元。特别是，5-羟色胺能神经元和髓鞘形成胶质细胞的形成存在缺陷。此外，在突变胚胎的前脑中观察到ENS标记基因的异位表达。这些发现表明该基因在神经前体细胞的迁移和分化中起重要作用，其缺失会导致斑马鱼的ENS和CNS发育异常。
