Wang Xiaoqin, Li Guizhen, Ho Row Kyung
xDepartment of Chemistry and Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, Korea.
J Chromatogr Sci. 2017 Sep 1;55(8):866-871. doi: 10.1093/chromsci/bmx037.
Deep eutectic solvents (DES) were formed from choline chloride (ChCl). DES-modified polymer monolithic (DES-M), template molecular polymer monolithic and non-DES-M without a molecular template were synthesized in identical process. These polymer materials were characterized by field emission scanning electron microscopy and Fourier transform infrared spectroscopy. The significant selective adsorption properties of the polymers were assessed by an absorption capacity experiment and solid-phase extraction (SPE). The optimized extraction procedure was as follows: ultrasonic time (30 min), optimal solvent (ethanol) and liquid to material ratio (20 mL g-1). Under this condition, the amount of quercetin extracted from Ginkgo biloba was 290.8 mg g-1. The purification of G. biloba was achieved by the SPE process. Based on the results, DESs-based monolithic cartridges can be used for simple and efficient extraction and as a pre-concentration technique for the purification of bioactive compounds or drugs in aqueous environments with high affinity and selectivity.
深共熔溶剂（DES）由氯化胆碱（ChCl）形成。在相同工艺中合成了DES改性聚合物整体柱（DES-M）、模板分子聚合物整体柱和无分子模板的非DES-M。通过场发射扫描电子显微镜和傅里叶变换红外光谱对这些聚合物材料进行了表征。通过吸附容量实验和固相萃取（SPE）评估了聚合物显著的选择性吸附性能。优化的萃取程序如下：超声时间（30分钟）、最佳溶剂（乙醇）和液料比（20 mL g-1）。在此条件下，从银杏叶中提取的槲皮素量为290.8 mg g-1。通过SPE工艺实现了银杏叶的纯化。基于这些结果，基于DES的整体柱可用于简单高效的萃取，并作为一种预浓缩技术，用于在水性环境中以高亲和力和选择性纯化生物活性化合物或药物。
