Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, Republic of Korea.
J Pharm Biomed Anal. 2011 Dec 5;56(4):713-20. doi: 10.1016/j.jpba.2011.07.014. Epub 2011 Jul 22.
Polyethyleneimine (PEI) and ethylenediamine (EDA) as modifiers were bonded on active carbon (AC) surface for specific selective extraction of quercetin from Oldenlandia diffusa. The characteristics of the modified AC materials that were obtained were investigated by field emission-scanning electron microscopy (FE-SEM) and Fourier transform infrared spectrometer (FT-IR). The interactions between quercetin and the AC materials were investigated by fitting the static adsorption data to four linear and nonlinear adsorption isotherm models. Of these four models, the Langmuir-Freundlich adsorption isotherm was proved the best for investigating quercetin on AC materials. Scatchard analysis was used to evaluate the binding properties of the AC materials for quercetin. Solvent extraction and solid-phase extraction (SPE) were optimized, and the effect of the mobile phase pH was investigated to improve the performance for the separation of quercetin on high performance liquid chromatography (HPLC). The results from the validation of the proposed analytical method demonstrated that the EDA-modified AC was the most suitable SPE cartridge for the purification of quercetin from O. diffusa.
聚乙烯亚胺（PEI）和乙二胺（EDA）被键合在活性炭（AC）表面上，用于从菊苣中特异性选择性提取槲皮素。通过场发射扫描电子显微镜（FE-SEM）和傅里叶变换红外光谱（FT-IR）研究了所得改性 AC 材料的特性。通过将静态吸附数据拟合到四个线性和非线性吸附等温线模型来研究槲皮素与 AC 材料之间的相互作用。在这四个模型中，Langmuir-Freundlich 吸附等温线被证明是研究 AC 材料上槲皮素的最佳模型。Scatchard 分析用于评估 AC 材料对槲皮素的结合特性。优化了溶剂萃取和固相萃取（SPE），并研究了流动相 pH 值的影响，以改善高效液相色谱（HPLC）中槲皮素分离的性能。所提出分析方法验证的结果表明，EDA 改性 AC 是从菊苣中纯化槲皮素最适合的 SPE 卡盒。
