Okada A, Bank I, Rogozinski L, Takihara Y, Mak T W, Chess L, Alt F W
Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, New York, New York 10032.
J Exp Med. 1988 Oct 1;168(4):1481-6. doi: 10.1084/jem.168.4.1481.
The CD3+, IL-2-dependent normal human thymocyte clone, CII, expresses on its surface a CD3-associated gamma/delta TCR. We have further elucidated the structure of this receptor from the nucleotide sequence of cDNA and genomic clones from CII that encode functional TCR-gamma and -delta chains. We find that the CII line expresses a C gamma 2 constant region that is a polymorphic form lacking a copy of an internal exon; the sequence of this constant region accounts for the size of the gamma chain and noncovalent linkage of gamma and delta chains in the CII TCR. The V gamma region used for the CII TCR is identical to the several previously characterized expressed human V gamma segments. Possible implications of this finding are discussed.
依赖白细胞介素-2的正常人类胸腺细胞克隆CII，其表面表达与CD3相关的γ/δT细胞受体。我们从编码功能性TCR-γ和-δ链的CII cDNA和基因组克隆的核苷酸序列中进一步阐明了该受体的结构。我们发现CII细胞系表达一种Cγ2恒定区，它是一种多态形式，缺少一个内部外显子拷贝；该恒定区的序列决定了γ链的大小以及CII TCR中γ链和δ链的非共价连接。用于CII TCR的Vγ区与几个先前已鉴定的表达的人类Vγ片段相同。讨论了这一发现的可能意义。
