Zhang Qi, Qu Qiaoyu, Chen Shanshan, Liu Xiaowei, Li Peiwu
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China.
Laboratory of Environmental Factors Risk Assessment of Agro-product Quality Safety, Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin 300191, PR China.
Food Chem. 2017 Sep 15;231:295-300. doi: 10.1016/j.foodchem.2017.02.016. Epub 2017 Feb 10.
A rapid and quantitative time-resolved fluorescent immunochromatographic assay (TRFICA) for detecting carbofuran residues in agro-products was reported in this paper. This assay was developed based on double-label immunoprobes, one of which was a carbofuran-specific antibody coupled with europium microbeads for the test (T) line signal while the other was mouse IgG coupled with europium microbeads for the control (C) line signal. Quantitative relationships between carbofuran concentrations and T/C ratios were established to determine the analyte concentration. To increase assay accuracy, four standard curves were established for the agro-products (green bean, cabbage, apple, and pear). The limits of detection (LODs) ranged from 0.04 to 0.76mgL. The spiked recoveries of carbofuran in the agro-products were in the range of 81-103%, which was in good agreement with a standard HPLC method. Therefore, we provided a new and reliable method for determination of N-methylcarbamate pesticide carbofuran residues in agro-products including vegetables and fruits.
本文报道了一种用于检测农产品中呋喃丹残留的快速定量时间分辨荧光免疫层析分析方法（TRFICA）。该分析方法基于双标记免疫探针开发，其中一个是与铕微珠偶联的呋喃丹特异性抗体，用于检测（T）线信号，另一个是与铕微珠偶联的小鼠IgG，用于对照（C）线信号。建立了呋喃丹浓度与T/C比值之间的定量关系以确定分析物浓度。为提高分析准确性，针对农产品（绿豆、卷心菜、苹果和梨）建立了四条标准曲线。检测限（LOD）范围为0.04至0.76mg/L。农产品中呋喃丹的加标回收率在81-103%范围内，与标准HPLC方法吻合良好。因此，我们提供了一种新的可靠方法，用于测定包括蔬菜和水果在内的农产品中氨基甲酸甲酯类农药呋喃丹的残留量。
