Kong Bihe, Chen Lei, Kasahara Yasuhiro, Sumida Akihiro, Ono Kiyomi, Wild Jan, Nagatake Arata, Hatano Ryusuke, Hara Toshihiko
Institute of Low Temperature Science, Hokkaido University.
Soil Science Laboratory, Graduate School of Agriculture, Hokkaido University.
Microbes Environ. 2017 Jun 24;32(2):103-111. doi: 10.1264/jsme2.ME16154. Epub 2017 Apr 28.
In order to understand the relationships between understory bamboo and soil properties, we compared microbial community structures in the soil of a Betula ermanii boreal forest with Sasa kurilensis present and removed using high-throughput DNA sequencing. The presence of understory S. kurilensis strongly affected soil properties, including total carbon, total nitrogen, nitrate, and the C:N ratio as well as relative soil moisture. Marked differences were also noted in fungal and bacterial communities between plots. The relative abundance of the fungal phylum Ascomycota was 13.9% in the Sasa-intact plot and only 0.54% in the Sasa-removed plot. Among the Ascomycota fungi identified, the most prevalent were members of the family Pezizaceae. We found that the abundance of Pezizaceae, known to act as mycorrhizal fungi, was related to the amount of total carbon in the Sasa-intact plot. The relative abundance of Proteobacteria was significantly higher, whereas those of Planctomycetes and Actinobacteria were lower in the Sasa-intact plot than in the Sasa-removed plot. Furthermore, the results obtained suggest that some species of the phylum Planctomycetes are more likely to occur in the presence of S. kurilensis. Collectively, these results indicate that the presence of S. kurilensis affects microbial communities and soil properties in a B. ermanii boreal forest.
为了了解林下竹类与土壤性质之间的关系，我们使用高通量DNA测序技术，比较了存在库页薹草和去除库页薹草的岳桦北方森林土壤中的微生物群落结构。林下库页薹草的存在强烈影响土壤性质，包括总碳、总氮、硝酸盐、碳氮比以及相对土壤湿度。不同样地之间的真菌和细菌群落也存在显著差异。在有库页薹草的样地中，子囊菌门真菌的相对丰度为13.9%，而在去除库页薹草的样地中仅为0.54%。在已鉴定的子囊菌门真菌中，最常见的是盘菌科成员。我们发现，已知作为菌根真菌的盘菌科的丰度与有库页薹草样地中的总碳量有关。在有库页薹草的样地中，变形菌门的相对丰度显著更高，而浮霉菌门和放线菌门的相对丰度则低于去除库页薹草的样地。此外，所得结果表明，浮霉菌门的一些物种更有可能在有库页薹草的情况下出现。总体而言，这些结果表明库页薹草的存在会影响岳桦北方森林中的微生物群落和土壤性质。
