Du Can, Xu Chen-Yang, Wang Qiang, Zhang Fan, Ma Wu-Gong, He Wen-Xiang, Hou Lin, Geng Zeng-Chao
Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture, College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China.
Forestry Bureau of Xinjiashan, Baoji 721700, China.
Huan Jing Ke Xue. 2017 Jul 8;38(7):3010-3019. doi: 10.13227/j.hjkx.201611002.
In this study, vertical changes in bacterial α-diversity and community composition were investigated at four soil depths(0-10, 10-20, 20-40 and 40-60 cm) in Burkill forest of Qinling Mountains by sequencing of the 16S rDNA regions using Illumina MiSeq high-throughput technology. The results showed that the decreases of OTUs, Chao1 and Shannon were numerical but not significant, and the highest values of 1688, 2314 and 8.66 were obtained in 0-10 cm, respectively. At the phylum level, Acidobacteria and Proteobacteria were the most dominant bacteria in four soil layers. At the genus level, and were the most dominant bacteria. The relative abundance of Acidobacteria in 40-60 cm soil depth(62.88%) was higher than those in other soil depths. Proteobacteria in 0-10 cm(23.62%) was more abundant than that in 40-60 cm. The relative abundance of Acidobacteria was significantly correlated with the total N, soil organic carbon, C/N, and soil dissolved organic carbon. Soil water content, soil organic matter and soil dissolved organic carbon were the key factors affecting soil Proteobacteria. RDA sequencing results showed that soil dissolved organic carbon was the key factor contributing to the bacteria community abundance. The results demonstrated that there are plenty of bacterial distribution in all four soil layers, which provides a fundamental basis for vertical soil bacterial community diversity, and possesses very important research value in biogeochemical cycling.
本研究采用Illumina MiSeq高通量技术对秦岭Burkill森林四个土壤深度（0 - 10、10 - 20、20 - 40和40 - 60厘米）的细菌α多样性和群落组成的垂直变化进行了调查。结果表明，OTU、Chao1和Shannon指数有所下降，但数值不显著，在0 - 10厘米处分别获得了最高值1688、2314和8.66。在门水平上，酸杆菌门和变形菌门是四个土壤层中最主要的细菌。在属水平上，[此处原文缺失具体属名]是最主要的细菌。40 - 60厘米土壤深度处酸杆菌门的相对丰度（62.88%）高于其他土壤深度。0 - 10厘米处变形菌门（23.62%）的丰度高于40 - 60厘米处。酸杆菌门的相对丰度与全氮、土壤有机碳、碳氮比和土壤溶解有机碳显著相关。土壤含水量、土壤有机质和土壤溶解有机碳是影响土壤变形菌门的关键因素。RDA测序结果表明，土壤溶解有机碳是影响细菌群落丰度的关键因素。结果表明，所有四个土壤层中都存在大量细菌分布，这为土壤细菌群落垂直多样性提供了基础依据，在生物地球化学循环方面具有非常重要的研究价值。
