Ruiz A M, López I V, López S, Espejo R T, Arias C F
Departamento de Biologia Molecular, Centro de Investigaciones sobre Ingenieria Genetica y Biotecnologia, Cuernavaca, Morelos, Mexico.
J Virol. 1988 Nov;62(11):4331-6. doi: 10.1128/JVI.62.11.4331-4336.1988.
In 1983, we isolated a porcine rotavirus (strain YM) that was prevalent in several regions of Mexico, as judged by the frequency of its characteristic electropherotype. By a focus reduction neutralization test, rotavirus YM was clearly distinguished from prototype rotavirus strains belonging to serotypes 1 (Wa), 2 (S2), 3 (SA11), 4 (ST3), 5 (OSU), and 6 (NCDV). Minor, one-way cross-neutralization (1 to 5%) was observed when antisera to the various rotavirus strains were incubated with rotavirus YM. In addition, the YM virus was not neutralized by neutralizing monoclonal antibodies with specificity to serotypes 1, 2, 3, and 5. The subgroup of the virus was determined to be I by enzyme-linked immunosorbent assay. To characterize the serotype-specific glycoprotein of the virus at the molecular level, we cloned and sequenced the gene coding for VP7. Comparison of the deduced amino acid sequence with reported homologous sequences from human and animal rotavirus strains belonging to six different serotypes further supported the distinct immunological identity of the YM VP7 protein.
1983年，我们分离出一株猪轮状病毒（YM株），根据其特征性电泳图谱的出现频率判断，该病毒在墨西哥的几个地区流行。通过蚀斑减少中和试验，YM轮状病毒与属于血清型1（Wa）、2（S2）、3（SA11）、4（ST3）、5（OSU）和6（NCDV）的原型轮状病毒株明显不同。当将各种轮状病毒株的抗血清与YM轮状病毒一起孵育时，观察到轻微的单向交叉中和（1%至5%）。此外，YM病毒不能被对血清型1、2、3和5具有特异性的中和单克隆抗体中和。通过酶联免疫吸附试验确定该病毒的亚组为I。为了在分子水平上表征该病毒的血清型特异性糖蛋白，我们克隆并测序了编码VP7的基因。将推导的氨基酸序列与来自属于六种不同血清型的人和动物轮状病毒株的报道同源序列进行比较，进一步支持了YM VP7蛋白独特的免疫特性。
