Clark H F, Hoshino Y, Bell L M, Groff J, Hess G, Bachman P, Offit P A
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
J Clin Microbiol. 1987 Sep;25(9):1757-62. doi: 10.1128/jcm.25.9.1757-1762.1987.
A virus (strain WI61) representing a presumptive new human serotype was isolated from an 18-month-old child with gastroenteritis admitted to Children's Hospital of Philadelphia in February 1983. The WI61 virus was clearly distinguished by cross-neutralization tests from human rotaviruses of serotypes 1, 2, 3, and 4, human 69M, and representative bovine (NCDV), porcine (OSU), and chicken (Ch2) rotaviruses. Antisera generated in guinea pigs hyperimmunized to WI61 virus displayed a partial cross-reactivity with rotaviruses of human serotypes 1, 2, 3, and 4. By means of studies with reassortant rotaviruses, it was presumptively determined that the WI61 virus cross-reactive antigenic determinants are localized on the vp3 surface polypeptide coded by gene segment 4. The characteristic RNA genome electropherotype of WI61 virus was observed in 5 of 59 cases of infant gastroenteritis detected in 1983 and 1984 but has not been observed in a subsequently at Children's Hospital. Serotype WI61-specific neutralizing antibodies were observed in a majority of sera of normal adults and infants of less than 4 or greater than 12 months of age collected in the Philadelphia area. Median antibody titers to WI61 equaled or exceeded those to rotaviruses of serotype 1 or 3. Each of seven samples of commercial cow's milk exhibited neutralizing antibodies to WI61 virus at a titer greater than or equal to that to serotype 1 or 3 or bovine (strain NCDV) rotavirus. However, WI61 rotavirus did not induce disease or a specific serum-neutralizing antibody response when fed to a caesarean-derived colostrum-deprived newborn calf. WI61 rotavirus caused diarrhea in newborn mice with a 50% diarrhea-inducing dose of 10(7.0) PFU.
1983年2月，从一名入住费城儿童医院的18个月大患肠胃炎的儿童体内分离出一种病毒（WI61株），它可能代表一种新的人类血清型。通过交叉中和试验，WI61病毒与血清型1、2、3和4的人类轮状病毒、人类69M以及代表性的牛（NCDV）、猪（OSU）和鸡（Ch2）轮状病毒有明显区别。用WI61病毒对豚鼠进行超免疫产生的抗血清与血清型1、2、3和4的人类轮状病毒表现出部分交叉反应性。通过对重配轮状病毒的研究，初步确定WI61病毒的交叉反应性抗原决定簇位于由基因片段4编码的vp3表面多肽上。在1983年和1984年检测的59例婴儿肠胃炎病例中，有5例观察到了WI61病毒特有的RNA基因组电泳图谱，但此后在儿童医院未再观察到。在费城地区收集的大多数正常成年人以及4个月以下或12个月以上婴儿的血清中，观察到了WI61血清型特异性中和抗体。针对WI61的抗体滴度中位数等于或超过针对血清型1或3轮状病毒的滴度。七个市售牛奶样本中的每一个都表现出针对WI61病毒的中和抗体，其滴度大于或等于针对血清型1或3或牛（NCDV株）轮状病毒的滴度。然而，将WI61轮状病毒喂给剖腹产出生且初乳缺乏的新生小牛时，并未引发疾病或产生特异性血清中和抗体反应。WI61轮状病毒可使新生小鼠腹泻，50%致泻剂量为10(7.0) PFU。
