Chen Qianru, Hou Hu, Wang Shikai, Zhao Xue, Li Bafang
College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
Food Funct. 2017 May 24;8(5):1933-1941. doi: 10.1039/c7fo00181a.
The effect of early enteral nutrition (EN) supplemented with Alaska pollock skin-derived collagen peptides (CPs) on post-burn inflammatory responses was investigated in a mouse model. Male blab/c mice were randomly assigned to four groups: a sham burn (SB) group, a control group (burn + EN + glycine, BE), a positive control group (burn + EN + glutamine, BEG) and a treatment group (burn + EN + CPs, BEC). Burn-induced increases of serum endotoxin level, and systemic and intestinal concentration of TNF-α and IL-6 were attenuated in BEG and BEC at post-burn day (PBD) 1, 3 and 7 (p < 0.05 vs. BE). Notably, BEC revealed a prominent decrease of the serum endotoxin level, TNF-α and IL-6 as compared to BEG at PBD 7 (p < 0.05). Furthermore, EN supplemented with CPs diminished the phosphorylation of intestinal NF-κB p65 and simultaneously down-regulated the mRNA expression of TNF-α and IL-6 in small intestine (p < 0.05 vs. BE). Also, it demonstrated a comparable effect with glutamine in ameliorating post-burn inflammatory responses in mice with burns. Therefore, CPs could be considered as a potential immunonutrient supplement in EN to improve post-burn outcomes in burn patients.
在小鼠模型中研究了早期肠内营养（EN）补充阿拉斯加狭鳕鱼皮源胶原蛋白肽（CPs）对烧伤后炎症反应的影响。雄性BALB/c小鼠被随机分为四组：假烧伤（SB）组、对照组（烧伤+EN+甘氨酸，BE）、阳性对照组（烧伤+EN+谷氨酰胺，BEG）和治疗组（烧伤+EN+CPs，BEC）。在烧伤后第1、3和7天，BEG组和BEC组烧伤诱导的血清内毒素水平、全身和肠道TNF-α及IL-6浓度升高均得到缓解（与BE组相比，p<0.05）。值得注意的是，在烧伤后第7天，BEC组的血清内毒素水平、TNF-α和IL-6与BEG组相比显著降低（p<0.05）。此外，补充CPs的EN减少了肠道NF-κB p65的磷酸化，同时下调了小肠中TNF-α和IL-6的mRNA表达（与BE组相比，p<0.05）。在改善烧伤小鼠的烧伤后炎症反应方面，它还显示出与谷氨酰胺相当的效果。因此，CPs可被视为EN中一种潜在的免疫营养补充剂，以改善烧伤患者的烧伤后结局。
