University of Illinois at Urbana-Champaign, Department of Mechanical Science and Engineering, Urbana, Illinois, United StatesbUniversity of Illinois at Urbana-Champaign, PROBE Lab, Urbana, Illinois, United States.
University of Illinois at Urbana-Champaign, Department of Mechanical Science and Engineering, Urbana, Illinois, United States.
J Biomed Opt. 2017 Apr 1;22(4):46009. doi: 10.1117/1.JBO.22.4.046009.
We use second-harmonic generation (SHG) microscopy to quantitatively characterize collagen fiber crimping in the posterior cruciate ligament (PCL). The obtained SHG images are utilized to define three distinct categories of crimp organization in the PCL. Using our previously published spatial-frequency analysis, we develop a simple algorithm to quantitatively distinguish the various crimp patterns. In addition, SHG microscopy reveals both the three-dimensional structural variation in some PCL crimp patterns as well as an underlying helicity in these patterns that have mainly been observed using electron microscopy. Our work highlights how SHG microscopy could potentially be used to link the fibrous structural information in the PCL to its mechanical properties.
我们使用二次谐波产生（SHG）显微镜定量描述后交叉韧带（PCL）中的胶原纤维卷曲。获得的 SHG 图像用于定义 PCL 中三种不同的卷曲组织。使用我们之前发表的空间频率分析，我们开发了一种简单的算法来定量区分各种卷曲模式。此外，SHG 显微镜揭示了一些 PCL 卷曲模式中的三维结构变化，以及这些模式中的螺旋结构，这些结构主要通过电子显微镜观察到。我们的工作强调了 SHG 显微镜如何将 PCL 的纤维结构信息与其机械性能联系起来。
