Parmaksiz Mahmut, Elçin Ayşe Eser, Elçin Yaşar Murat
Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University, Faculty of Science and Stem Cell Institute, Ankara, Turkey.
Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara University, Faculty of Science, Ankara, Turkey.
Methods Mol Biol. 2019;1879:175-185. doi: 10.1007/7651_2018_147.
Tissue engineering-based regenerative applications can involve the use of stem cells for the treatment of non-healing wounds. Multipotent mesenchymal stem cells have become a focus of skin injury treatments along with many other injury types owing to their unprecedented advantages. However, there are certain limitations concerning the solo use of stem cells in skin wound repair. Natural bioactive extracellular matrix-based scaffolds have great potential for overcoming these limitations by supporting the regenerative activity and localization of stem cells. This chapter describes the use of bone marrow mesenchymal stem cells together with decellularized bovine small intestinal submucosa (SIS), for the treatment of a critical-sized full-thickness skin defect in a small animal model.
基于组织工程的再生应用可涉及使用干细胞治疗难愈合伤口。多能间充质干细胞因其前所未有的优势，已成为皮肤损伤以及许多其他损伤类型治疗的焦点。然而，在皮肤伤口修复中单独使用干细胞存在一定局限性。基于天然生物活性细胞外基质的支架在支持干细胞的再生活性和定位方面具有巨大潜力，有望克服这些局限性。本章描述了在小动物模型中，将骨髓间充质干细胞与去细胞牛小肠黏膜下层（SIS）联合使用，用于治疗临界尺寸的全层皮肤缺损。
