Comanducci M, Ricci S, Ratti G
Sclavo Research Centre, Siena, Italy.
Mol Microbiol. 1988 Jul;2(4):531-8. doi: 10.1111/j.1365-2958.1988.tb00060.x.
Sequence analysis of a 7.5 kb DNA plasmid isolated from Chlamydia trachomatis shows 8 open reading frames (ORFs) regularly spaced along most of the sequence. One of these ORFs encodes a 451-amino-acid polypeptide highly homologous to the DnaB protein of Escherichia coli. A region between ORFs 6 and 7 contains a cluster of alternating ATs and a 22 bp sequence tandemly repeated 4 times, suggesting a replication control region. Several ORFs correspond to plasmid-specific polypeptides that have been described. Codons ending with A or T are more frequent, as might be expected from the high A/T content (64%) of the plasmid, and codon usage is similar to that of the C. trachomatis chromosomal gene, omp1L2.
对从沙眼衣原体中分离出的一个7.5 kb DNA质粒进行序列分析，结果显示在大部分序列上有8个开放阅读框（ORF）规则排列。其中一个ORF编码一种451个氨基酸的多肽，它与大肠杆菌的DnaB蛋白高度同源。在ORF 6和7之间的一个区域包含一串交替出现的AT以及一段22 bp的序列，该序列串联重复4次，提示这是一个复制控制区域。有几个ORF对应于已描述的质粒特异性多肽。以A或T结尾的密码子更为常见，这正如从该质粒较高的A/T含量（64%）所预期的那样，并且密码子使用情况与沙眼衣原体染色体基因omp1L2相似。
