Palmer L, Falkow S
Plasmid. 1986 Jul;16(1):52-62. doi: 10.1016/0147-619x(86)90079-x.
A 7.4-kb plasmid is a common and perhaps essential component of the Chlamydia trachomatis genome. This plasmid occurs as 10 copies per chlamydial chromosomal equivalent. It is unable to replicate in Escherichia coli. Complete plasmid genomes from eight serovars of C. trachomatis have been isolated in E. coli as cloned sequences ligated to pBR322. Restriction enzyme cleavage site mapping indicates that these plasmids are closely related. Homologous plasmid sequences have also been detected by DNA hybridization in all of the 200 clinically isolated strains of C. trachomatis which have been examined. DNA sequences homologous to the C. trachomatis plasmid were not found in eucaryotic DNA nor in a plasmid of similar size isolated from C. psittaci. C. trachomatis plasmid genes are expressed in vivo and the plasmid encoded gene products may play a role in the intracellular growth of this organism. Plasmid encoded genes were also expressed from the cloned C. trachomatis plasmid in E. coli minicells and using an E. coli S-30 in vitro transcription translation extract.
一个7.4千碱基的质粒是沙眼衣原体基因组常见且可能必不可少的组成部分。该质粒以每个衣原体染色体等价物10个拷贝的形式存在。它无法在大肠杆菌中复制。来自沙眼衣原体8个血清型的完整质粒基因组已作为与pBR322连接的克隆序列在大肠杆菌中分离出来。限制性内切酶切割位点图谱表明这些质粒密切相关。在所有已检测的200株临床分离的沙眼衣原体菌株中，通过DNA杂交也检测到了同源质粒序列。在真核DNA中或从鹦鹉热衣原体分离的大小相似的质粒中均未发现与沙眼衣原体质粒同源的DNA序列。沙眼衣原体质粒基因在体内表达，质粒编码的基因产物可能在该生物体的细胞内生长中起作用。质粒编码的基因也在大肠杆菌小细胞中从克隆的沙眼衣原体质粒表达，并使用大肠杆菌S-30体外转录翻译提取物进行表达。
