Li Lei, Wang Wei, Zhang Renya, Liu Jianli, Yu Juan, Wu Xiaoxiao, Xu Ying, Ma Ming, Huang Jian
Department of Pathology, Affiliated Hospital of Jining Medical University, Jining, Shandong, China.
Thoracic Surgery, Affiliated Hospital of Jining Medical University, Jining, Shandong, China.
Cancer Biomark. 2017 Jul 4;19(3):305-311. doi: 10.3233/CBM-160469.
LAMP2 is one of the major protein components of lysosome. In addition to the expression on the lysosomal membrane, LAMP2 has also been found relocalizing to the cell surface of some highly metastatic tumor cells.
The aim of this study was to detect the expression levels of LAMP2 and discuss its roles in esophageal squamous cell carcinoma (ESCC).
Six hundred and ten tissue samples of ESCC were collected to construct tissue microarrays, which were stained by immunohistochemistry.
After immunohistochemical staining, 596 patients including 460 men and 136 women were analyzed. The LAMP2 expression levels were significantly different based on degrees of histological differentiation (χ2= 108.906, P< 0.001). The similar results were also observed in TNM stages (χ2= 23.835, P< 0.01). LAMP2 expression levels negatively correlated with degrees of histological differentiation (P< 0.01). Logistic regression analysis showed that the LAMP2 expression levels were correlated with the degrees of histological differentiation (OR=𝑑𝑖𝑓𝑓𝑒𝑟𝑒𝑛𝑡𝑖𝑎𝑡𝑖𝑜𝑛 0.452, P< 0.001) and TNM stages (OR=𝑇𝑁𝑀 1.482, P= 0.42). Besides, Kaplan-Meier survival curves indicated that patients with higher expression of LAMP2 exhibited poor prognosis (P< 0.05).
Our results demonstrated that LAMP2 expression levels correlated with tumor histological differentiation and TNM stages. High expression of LAMP2 predicts poor prognosis in patients with ESCC.
LAMP2是溶酶体的主要蛋白质成分之一。除了在溶酶体膜上表达外，还发现LAMP2重新定位到一些高转移肿瘤细胞的细胞表面。
本研究旨在检测LAMP2的表达水平，并探讨其在食管鳞状细胞癌（ESCC）中的作用。
收集610例ESCC组织样本构建组织芯片，采用免疫组织化学染色。
免疫组织化学染色后，分析了596例患者，其中男性460例，女性136例。LAMP2表达水平根据组织学分化程度有显著差异（χ2 = 108.906，P < 0.001）。在TNM分期中也观察到类似结果（χ2 = 23.835，P < 0.01）。LAMP2表达水平与组织学分化程度呈负相关（P < 0.01）。Logistic回归分析显示，LAMP2表达水平与组织学分化程度相关（OR =分化0.452，P < 0.001）和TNM分期（OR = TNM 1.482，P = 0.42）。此外，Kaplan-Meier生存曲线表明，LAMP2表达较高的患者预后较差（P < 0.05）。
我们的结果表明，LAMP2表达水平与肿瘤组织学分化和TNM分期相关。LAMP2高表达预示ESCC患者预后不良。
