Furano A V, Robb S M, Robb F T
Section on Genomic Structure and Function, National Institute of Diabetes, and Digestive and Kidney Diseases, Bethesda, MD 20892.
Nucleic Acids Res. 1988 Oct 11;16(19):9215-31. doi: 10.1093/nar/16.19.9215.
Here we report the DNA structure of the left 1.5 kb of two newly isolated full length members of the rat L1 DNA family (L1Rn, long interspersed repeated DNA). In contrast to earlier isolated rat L1 members, both of these contain promoter-like regions that are most likely full length. In addition, the promoter-like region of both members has undergone a partial tandem duplication. A second internal region of the left end of one of the reported members is also tandemly duplicated. The propensity of the left end of rat L1 elements to undergo this form of genetic rearrangement, as well as other structural features revealed by the present work, is discussed in light of the fact that during evolution the otherwise conserved mammalian L1 DNA families have each acquired completely different promoter-like regions. In an accompanying paper [Nur, I., Pascale, E., and Furano, A. V. (1988) Nucleic Acids Res. 16, submitted], we report that one of the rat promoter-like regions can function as a promoter in rat cells when fused to the Escherichia coli chloramphenicol acyltransferase gene.
在此我们报道了大鼠L1 DNA家族（L1Rn，长散在重复DNA）两个新分离的全长成员左侧1.5 kb的DNA结构。与早期分离的大鼠L1成员不同，这两个成员均含有很可能是全长的启动子样区域。此外，两个成员的启动子样区域都经历了部分串联重复。所报道的一个成员左端的第二个内部区域也发生了串联重复。鉴于在进化过程中，原本保守的哺乳动物L1 DNA家族各自获得了完全不同的启动子样区域这一事实，我们讨论了大鼠L1元件左端发生这种基因重排形式的倾向以及本研究揭示的其他结构特征。在一篇配套论文中[Nur, I., Pascale, E., and Furano, A. V. (1988) Nucleic Acids Res. 16, submitted]，我们报道当与大肠杆菌氯霉素酰基转移酶基因融合时，大鼠的一个启动子样区域可在大鼠细胞中作为启动子发挥作用。
