Grimaldi G, Skowronski J, Singer M F
EMBO J. 1984 Aug;3(8):1753-9. doi: 10.1002/j.1460-2075.1984.tb02042.x.
Comparison of the sequences at the ends of several newly cloned and full length members of the monkey KpnI family with one another and with previously described monkey and human segments defines the nucleotide sequence at the two termini. No terminal repeats either direct or inverted are noted within full length family members which may or may not be immediately flanked by direct repeats. At the 3' terminus, several family members have polyadenylation signals followed by a d(A)-rich stretch. The genomic frequency of segments within the full length element increases markedly from the 5' to the 3' terminus, consistent with the cloning of various truncated family members. One such truncated version joined to a low copy number DNA segment is inserted in monkey alpha-satellite where the combination appears to have been amplified in conjunction with the satellite itself.
将几个新克隆的猴KpnI家族全长成员末端的序列相互比较，并与先前描述的猴和人类片段进行比较，确定了两个末端的核苷酸序列。在全长家族成员中未发现正向或反向的末端重复序列，这些成员可能紧邻也可能不紧邻正向重复序列。在3'末端，几个家族成员具有多聚腺苷酸化信号，随后是富含d(A)的片段。全长元件内片段的基因组频率从5'末端到3'末端显著增加，这与各种截短家族成员的克隆结果一致。一个这样的截短版本与一个低拷贝数DNA片段相连，插入到猴α卫星中，在那里这种组合似乎与卫星本身一起被扩增。
