Cotecchia S, Schwinn D A, Randall R R, Lefkowitz R J, Caron M G, Kobilka B K
Department of Medicine, Duke University Medical Center, Durham, NC 27710.
Proc Natl Acad Sci U S A. 1988 Oct;85(19):7159-63. doi: 10.1073/pnas.85.19.7159.
The cDNA for the Syrian hamster alpha 1-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the receptor protein purified from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes a 515-residue polypeptide that shows the most sequence identity with the other adrenergic receptors and the putative protein product of the related clone G-21. Similarities with the muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells confirm that we have cloned the alpha 1-adrenergic receptor that couples to inositol phospholipid metabolism.
利用与从DDT1MF-2平滑肌细胞纯化的受体蛋白部分氨基酸序列相对应的寡核苷酸，克隆了叙利亚仓鼠α1-肾上腺素能受体的cDNA。推导的氨基酸序列编码一个515个残基的多肽，该多肽与其他肾上腺素能受体以及相关克隆G-21的推定蛋白质产物具有最高的序列同一性。与毒蕈碱型胆碱能受体的相似性也很明显。在COS-7细胞中的表达研究证实，我们克隆到了与肌醇磷脂代谢偶联的α1-肾上腺素能受体。
