Yeow Natasha, Tabor Rico F, Garnier Gil
Bioresource Processing Research Institute Australia (BioPRIA), Department of Chemical Engineering, Monash University, Clayton, VIC 3800, Australia.
School of Chemistry, Monash University, Clayton, VIC 3800, Australia.
Colloids Surf B Biointerfaces. 2017 Jul 1;155:373-378. doi: 10.1016/j.colsurfb.2017.04.038. Epub 2017 Apr 21.
Most blood grouping tests rely on the principle of red blood cells (RBCs) agglutination. Agglutination is triggered by the binding of specific blood grouping antibodies to the corresponding RBC surface antigen on multiple cells. The interaction energies between blood grouping antibodies and antigens have been poorly defined in immunohaematology. Here for the first time, we functionalized atomic force microscope (AFM) cantilevers with the IgM form of blood grouping antibodies to probe populations of individual RBCs of different groups under physiological conditions. The force-mapping mode of AFM allowed us to measure specific antibody - antigen interactions, and simultaneously localize and quantify antigen sites on the scanned cell surface. This study provides a new insight of the interactions between IgM antibodies and its corresponding antigen. The technique and information can be translated to develop better blood typing diagnostics and optimize target-specific drug delivery for medical applications.
大多数血型检测依赖于红细胞（RBC）凝集原理。凝集是由特定血型抗体与多个细胞上相应的红细胞表面抗原结合引发的。在免疫血液学中，血型抗体与抗原之间的相互作用能尚未得到很好的界定。在此，我们首次用IgM形式的血型抗体对原子力显微镜（AFM）悬臂进行功能化，以在生理条件下探测不同血型的单个红细胞群体。AFM的力映射模式使我们能够测量特异性抗体 - 抗原相互作用，并同时定位和量化扫描细胞表面上的抗原位点。本研究为IgM抗体与其相应抗原之间的相互作用提供了新的见解。该技术和信息可转化用于开发更好的血型诊断方法，并优化医疗应用中的靶向药物递送。
