Guo Qingqu
Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China.
Oncol Lett. 2017 Mar;13(3):1575-1580. doi: 10.3892/ol.2017.5613. Epub 2017 Jan 18.
Mitochondrial dysfunction is linked to cancer. Differences in the number, morphology and function of mitochondria have been observed between normal cells and cancer cells. However, changes in mitochondrial function during epithelial-mesenchymal transition (EMT) in pancreatic cancer are less known. In the present study, the cultured human pancreatic cancer cell line Panc-1 was treated with transforming growth factor (TGF)β-1. Mitochondrial functions following TGFβ-1 exposure in pancreatic cancer were investigated. It was noticed that TGFβ-1 treatment induces morphologic changes and a shift from epithelial to mesenchymal phenotype in pancreatic cancer. Furthermore, increased mitochondrial mass was detected in pancreatic cancer following TGFβ-1 treatment. Besides, the production of reactive oxygen species in TGFβ-1-treated pancreatic cancer cells significantly increased compared with the control cells. Our results indicate that the phenomenon of EMT in pancreatic cancer has an association with mitochondrial dysfunction. Mitochondrial dysfunction may be a cause of EMT in pancreatic cancer, which leads to heterogeneity in pancreatic cancer, and may be a potential therapeutic target in the future.
线粒体功能障碍与癌症相关。正常细胞与癌细胞之间已观察到线粒体在数量、形态和功能上的差异。然而，胰腺癌上皮-间质转化（EMT）过程中线粒体功能的变化却鲜为人知。在本研究中，用转化生长因子（TGF）β-1处理培养的人胰腺癌细胞系Panc-1。研究了胰腺癌中TGFβ-1暴露后的线粒体功能。注意到TGFβ-1处理诱导了胰腺癌的形态变化以及从上皮表型向间质表型的转变。此外，TGFβ-1处理后的胰腺癌中线粒体质量增加。此外，与对照细胞相比，TGFβ-1处理的胰腺癌细胞中活性氧的产生显著增加。我们的结果表明，胰腺癌中的EMT现象与线粒体功能障碍有关。线粒体功能障碍可能是胰腺癌中EMT的一个原因，这导致了胰腺癌的异质性，并且可能是未来潜在的治疗靶点。
