EMT 信号转导：CRISPR/Cas 基因编辑的潜在贡献。
EMT signaling: potential contribution of CRISPR/Cas gene editing.
机构信息
Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Section of Experimental Pathology and Oncology, Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, Florence, Italy.
出版信息
Cell Mol Life Sci. 2020 Jul;77(14):2701-2722. doi: 10.1007/s00018-020-03449-3. Epub 2020 Feb 1.
Abstract
Epithelial to mesenchymal transition (EMT) is a complex plastic and reversible cellular process that has critical roles in diverse physiological and pathological phenomena. EMT is involved in embryonic development, organogenesis and tissue repair, as well as in fibrosis, cancer metastasis and drug resistance. In recent years, the ability to edit the genome using the clustered regularly interspaced palindromic repeats (CRISPR) and associated protein (Cas) system has greatly contributed to identify or validate critical genes in pathway signaling. This review delineates the complex EMT networks and discusses recent studies that have used CRISPR/Cas technology to further advance our understanding of the EMT process.
摘要
上皮间质转化（EMT）是一个复杂的、具有可塑性和可逆性的细胞过程，在多种生理和病理现象中起着关键作用。EMT 参与胚胎发育、器官发生和组织修复，以及纤维化、癌症转移和耐药性。近年来，使用成簇规律间隔短回文重复（CRISPR）和相关蛋白（Cas）系统编辑基因组的能力极大地促进了对途径信号中关键基因的鉴定或验证。本文阐述了复杂的 EMT 网络，并讨论了最近使用 CRISPR/Cas 技术的研究，这些研究进一步推进了我们对 EMT 过程的理解。
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