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微小RNA-215抑制非小细胞肺癌细胞的增殖和迁移。

miR-215 suppresses proliferation and migration of non-small cell lung cancer cells.

作者信息

Cai Xiaopan, Peng Dongyu, Wei Haifeng, Yang Xinghai, Huang Quan, Lin Zaijun, Xu Wei, Qian Ming, Yang Cheng, Liu Tielong, Yan Wangjun, Zhao Jian

机构信息

Department of Bone Tumor Surgery, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P.R. China.

出版信息

Oncol Lett. 2017 Apr;13(4):2349-2353. doi: 10.3892/ol.2017.5692. Epub 2017 Feb 7.

DOI:10.3892/ol.2017.5692
PMID:28454402
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5403480/
Abstract

The expression of microRNA-215 (miR-215) in non-small cell lung cancer (NSCLC) tissues and the effects of miR-215 on the proliferation and migration of NSCLC cells were investigated. qRT-PCR was used to detect the expression of miR-215 in NSCLC tissues and paired normal tumor-adjacent lung tissues; MTT assay, transwell assay and soft-agar assay were used to evaluate the role of miR-215 on proliferation, migration and cell clonality on NSCLC cells, after transfecting miR-215 mimics to NSCLC cell line A549 or miR-215 to H1299. miR-215 was significantly decreased in NSCLC tissues compared to the paired normal tissues; Overexpression of miR-215 in A549 cells resulted in reduction of the cell proliferation, migration and cell clonality, while downregulation of miR-215 in H1299 cells could promote cell proliferation, migration and clonality. In conclusion, miR-215 was downregulated in NSCLC tissues and may play a key role in the development of NSCLC.

摘要

研究了非小细胞肺癌（NSCLC）组织中微小RNA-215（miR-215）的表达以及miR-215对NSCLC细胞增殖和迁移的影响。采用qRT-PCR检测NSCLC组织及配对的肿瘤旁正常肺组织中miR-215的表达；在将miR-215模拟物转染至NSCLC细胞系A549或miR-215转染至H1299后，采用MTT法、Transwell法和软琼脂法评估miR-215对NSCLC细胞增殖、迁移和细胞克隆性的作用。与配对的正常组织相比，NSCLC组织中miR-215显著降低；A549细胞中miR-215的过表达导致细胞增殖、迁移和细胞克隆性降低，而H1299细胞中miR-215的下调可促进细胞增殖、迁移和克隆性。总之，miR-215在NSCLC组织中表达下调，可能在NSCLC的发生发展中起关键作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c58/5403480/43f1f327d914/ol-13-04-2349-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c58/5403480/868447884713/ol-13-04-2349-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c58/5403480/fa35a4110751/ol-13-04-2349-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c58/5403480/09e4c1293c5f/ol-13-04-2349-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c58/5403480/c754ab41bfa0/ol-13-04-2349-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c58/5403480/43f1f327d914/ol-13-04-2349-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c58/5403480/868447884713/ol-13-04-2349-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c58/5403480/fa35a4110751/ol-13-04-2349-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c58/5403480/09e4c1293c5f/ol-13-04-2349-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c58/5403480/c754ab41bfa0/ol-13-04-2349-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c58/5403480/43f1f327d914/ol-13-04-2349-g04.jpg

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