Oseguera-Toledo Miguel E, Gonzalez de Mejia Elvira, Amaya-Llano Silvia L
Programa de Posgrado en Alimentos del Centro de la República (PROPAC), Universidad Autónoma de Querétaro, Apdo. Postal 184, Querétaro, QRO 76010, Mexico; Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Food Res Int. 2015 Oct;76(Pt 3):839-851. doi: 10.1016/j.foodres.2015.07.046. Epub 2015 Aug 1.
The objective was to evaluate the effect of bioactive peptide fractions from de-hulled hard-to-cook (HTC) bean on enzyme targets of type-2 diabetes and oxidative stress. Protein isolates from Pinto Durango and Negro 8025 beans were hydrolyzed (120min) with either alcalase® or bromelain and separated into five peptide fractions (<1, 1-3.5, 3.5-5, 5-10, and >10kDa) using an ultrafiltration membrane system. The <1kDa pinto Durango-bromelain fraction showed the best inhibition of α-amylase (49.9±1.4%), and the <1kDa pinto Durango-alcalase fraction inhibited both, α-glucosidase (76.4±0.5%), and dipeptidyl peptidase-IV (DPP-IV, 55.3±1.6%). Peptides LLSL, QQEG and NEGEAH were present in the most potent fractions. Hydrolysates and peptide fractions showed antioxidant capacity (ORAC: 159.6±2.9 to 932.6±1.1mmolTE/g) and nitric oxide inhibition (57.5±0.9 to 68.3±4.2%). Hydrolysates and fractions <1 and 1-3kDa were able to increase glucose-stimulated insulin secretion from iNS-1E cells up to 57% compared to glucose control. Hydrolysates from HTC beans inhibited enzymes related to diabetes management, being the smallest peptides (<1kDa) the most potent. HTC bean could be a source of protein to produce bioactive peptides with potential antidiabetic properties.
目的是评估去皮难煮（HTC）豆中的生物活性肽组分对2型糖尿病酶靶点和氧化应激的影响。用碱性蛋白酶®或菠萝蛋白酶对杜兰戈花豆和Negro 8025豆的蛋白质分离物进行水解（120分钟），并使用超滤膜系统将其分离成五个肽组分（<1、1 - 3.5、3.5 - 5、5 - 10和>10 kDa）。<1 kDa的杜兰戈花豆 - 菠萝蛋白酶组分对α - 淀粉酶的抑制作用最佳（49.9±1.4%），<1 kDa的杜兰戈花豆 - 碱性蛋白酶组分对α - 葡萄糖苷酶（76.4±0.5%）和二肽基肽酶 - IV（DPP - IV，55. ,3±1.6%）均有抑制作用。最有效的组分中存在肽LLSL、QQEG和NEGEAH。水解产物和肽组分具有抗氧化能力（ORAC：159.6±2.9至932.6±1.1 mmol TE/g）和一氧化氮抑制能力（57.5±0.9至68.3±4.2%）。与葡萄糖对照组相比，<1和1 - 3 kDa的水解产物和组分能够使iNS - 1E细胞中葡萄糖刺激的胰岛素分泌增加高达57%。HTC豆的水解产物抑制与糖尿病管理相关的酶，最小的肽（<1 kDa）最为有效。HTC豆可能是生产具有潜在抗糖尿病特性的生物活性肽的蛋白质来源。
