Westbury H A, Doughty W J, Forman A J, Tangchaitrong S, Kongthon A
CSIRO, Australian Animal Health Laboratory, Geelong, Vic.
Vet Microbiol. 1988 May;17(1):21-8. doi: 10.1016/0378-1135(88)90076-4.
A total of 205 epithelial tissue samples were examined for the presence of foot and mouth disease virus by either the complement-fixation (CF) test, enzyme-linked immunosorbent assay (ELISA) and/or by virus isolation in bovine thyroid or kidney cell cultures. The virus was isolated from 134 of the 201 (67%) specimens. Samples, from which virus was isolated, were termed virus-positive samples. The CF test detected viral antigen in 30 (24%) of 123 virus-positive samples, whereas the ELISA detected it in 100 (81%) of these specimens. The ELISA was thus at least 3 times more efficient than the CF test in detecting the virus in epithelial-tissue samples. There were 5 samples from which virus was not isolated but which were positive with the ELISA procedure. The ELISA was particularly useful for testing samples from pigs and for assessing specimens from animals with resolving lesions. The ELISA gave virus type-specific results in 89% of 63 virus-positive cases compared to 40% for the CF test. The ELISA was thus a very useful, accurate and sensitive method for the direct testing of epithelial tissues of affected animals.
总共205份上皮组织样本通过补体结合（CF）试验、酶联免疫吸附测定（ELISA）和/或在牛甲状腺或肾细胞培养物中进行病毒分离来检测口蹄疫病毒的存在。在201份样本中的134份（67%）中分离出了病毒。分离出病毒的样本被称为病毒阳性样本。CF试验在123份病毒阳性样本中的30份（24%）中检测到了病毒抗原，而ELISA在这些样本中的100份（81%）中检测到了病毒抗原。因此，ELISA在上皮组织样本中检测病毒的效率至少是CF试验的3倍。有5份样本未分离出病毒，但ELISA检测呈阳性。ELISA对于检测猪的样本以及评估病变正在消退的动物的样本特别有用。在63例病毒阳性病例中，ELISA在89%的病例中给出了病毒型特异性结果，而CF试验为40%。因此，ELISA是一种非常有用、准确且灵敏的直接检测受感染动物上皮组织的方法。
