Meyling A, Jensen A M
National Veterinary Laboratory, Copenhagen, Denmark.
Vet Microbiol. 1988 Jun;17(2):97-105. doi: 10.1016/0378-1135(88)90001-6.
Twelve heifers that did not have antibodies to bovine virus diarrhoea virus (BVDV) were inseminated with semen from a bull that was persistently infected with the virus and contained 10(4.0)-10(6.5) TCID50 0.1 ml-1. All 12 became infected, as indicated by seroconversion within 2 weeks of insemination. Four control heifers were inseminated with virus-free semen. The virus was not transmitted to these animals in spite of close contact with the heifers inseminated with the infected semen. All the heifers became pregnant and gave birth to clinically normal calves at term. However, one calf was born persistently infected with BVDV. After the birth of this persistently-infected calf the control heifers and their calves seroconverted. The study demonstrates that BVDV may be transmitted in cattle by artificial insemination (AI). Therefore entry of persistently-infected animals into AI centres should be prevented.
12头未感染牛病毒性腹泻病毒（BVDV）抗体的小母牛用来自一头持续感染该病毒且每0.1毫升含有10⁴.⁰ - 10⁶.⁵半数组织培养感染剂量（TCID₅₀）的公牛精液进行人工授精。所有12头小母牛均被感染，授精后2周内血清转化表明了这一点。4头对照小母牛用无病毒精液进行人工授精。尽管与用感染精液授精的小母牛密切接触，但病毒并未传播给这些动物。所有小母牛均怀孕并足月产下临床正常的犊牛。然而，一头犊牛出生时持续感染BVDV。这头持续感染的犊牛出生后，对照小母牛及其犊牛血清转化。该研究表明，BVDV可能通过人工授精（AI）在牛群中传播。因此，应防止持续感染的动物进入人工授精中心。
