Sharma J M
United States Department of Agriculture, Regional Poultry Research Laboratory, East Lansing, MI 48823.
Vet Immunol Immunopathol. 1988 Jul;19(1):51-66. doi: 10.1016/0165-2427(88)90046-3.
Spleen cells but not the thymus or the bursa cells of chicken embryos suppressed the in vitro mitogenesis of spleen cells of adult syngeneic or allogeneic chickens. The natural suppressor cell activity of embryo spleen was present at embryonation day 16, reached peak levels at embryonation day 18 and disappeared at hatch. The embryo spleen cells did not by themselves respond to phytohemagglutinin stimulation in vitro. The suppressive effect of embryonic spleen cells on adult spleen cells was present when the embryonic cells were added at the time of or after initiation of the adult spleen mitogenic cultures. When the embryonic cells were added to the cultures of adult spleen cells after the blastogenic response of the adult cells had peaked, the embryonic cells inhibited the incorporation of the label into adult spleen cell blasts. The suppressive activity of the embryonic spleen cells was mediated by soluble suppressor product(s) secreted by these cells, and direct cell-to-cell contact between embryonic and adult spleen cells was not necessary for suppression to occur. Infection of embryos with turkey herpesvirus and Marek's disease virus reduced the suppressor cell activity of embryonic spleen, although substantial residual suppressor cell activity remained in virus-infected embryos. Several pathogenic or non-pathogenic isolates of infectious bursal disease virus did not appreciably alter the suppressor cell activity of embryonic spleen cells.
鸡胚的脾细胞而非胸腺或法氏囊细胞抑制成年同基因或异基因鸡脾细胞的体外有丝分裂。胚胎脾的天然抑制细胞活性在胚胎发育第16天出现，在胚胎发育第18天达到峰值水平，并在孵化时消失。胚胎脾细胞本身在体外对植物血凝素刺激无反应。当在成年脾有丝分裂培养开始时或之后加入胚胎细胞时，胚胎脾细胞对成年脾细胞具有抑制作用。当成年细胞的增殖反应达到峰值后将胚胎细胞加入成年脾细胞培养物中时，胚胎细胞会抑制标记物掺入成年脾细胞母细胞。胚胎脾细胞的抑制活性由这些细胞分泌的可溶性抑制产物介导，胚胎和成年脾细胞之间的直接细胞间接触对于抑制的发生并非必要。用火鸡疱疹病毒和马立克氏病病毒感染胚胎会降低胚胎脾的抑制细胞活性，不过在病毒感染的胚胎中仍保留大量残余抑制细胞活性。传染性法氏囊病病毒的几种致病性或非致病性分离株并未明显改变胚胎脾细胞的抑制细胞活性。
