Guan Xue, Zong Zhi-Hong, Chen Shuo, Sang Xiu-Bo, Wu Dan-Dan, Wang Li-Li, Liu Yao, Zhao Yang
Department of Gynecology, The First Affiliated Hospital of China Medical University, Shenyang 110001, China.
Department of Biochemistry and Molecular Biology, College of Basic Medicine, China Medical University, Shenyang 100013, China.
Gene. 2017 Aug 15;624:14-20. doi: 10.1016/j.gene.2017.04.043. Epub 2017 Apr 27.
MicroRNA-372 has been shown to be associated with multiple tumors' development and progression, by regulating the expression of proteins involved in cell cycle and apoptosis. However, the specific mechanism and function of miR-372 in ovarian carcinoma are not clear. Our study explored the role of miR-372 in ovarian carcinoma cell cycle and proliferation.
MiR-372 expression was quantified in normal ovarian tissue, benign tumors, primary ovarian carcinomas and metastatic omentum by qRT-PCR. MTT assay and plate clone formation assay were performed to evaluate the cell viability and proliferation. EDU assay and cell apoptosis assay were also used to determine cell growth. We used Western Blot to analysis expression of the known miR-372 targets.
We found that miR-372 expression was significantly lower in ovarian carcinoma than normal ovarian tissues and benign tumors. Moreover, miR-372 overexpression showed significant inhibition of cell proliferation and promoted cell apoptosis. Western Blot revealed that miR-372 downregulated the expression of ATAD2, LATS2, P62, DKK1 and cyclinA1 to inhibit the proliferation of cells.
Our findings indicate that miR-372 has a prominent role in inhibiting tumor growth and it is a valuable target for ovarian cancer therapy.
MicroRNA-372已被证明通过调节参与细胞周期和凋亡的蛋白质表达，与多种肿瘤的发生和发展相关。然而，miR-372在卵巢癌中的具体机制和功能尚不清楚。我们的研究探讨了miR-372在卵巢癌细胞周期和增殖中的作用。
通过qRT-PCR对正常卵巢组织、良性肿瘤、原发性卵巢癌和转移性大网膜中的miR-372表达进行定量。采用MTT法和平板克隆形成试验评估细胞活力和增殖。EDU试验和细胞凋亡试验也用于确定细胞生长情况。我们使用蛋白质免疫印迹法分析已知的miR-372靶标的表达。
我们发现卵巢癌中miR-372的表达明显低于正常卵巢组织和良性肿瘤。此外，miR-372过表达显著抑制细胞增殖并促进细胞凋亡。蛋白质免疫印迹法显示，miR-372下调ATAD2、LATS2、P62、DKK1和细胞周期蛋白A1的表达以抑制细胞增殖。
我们的研究结果表明，miR-372在抑制肿瘤生长方面具有重要作用，是卵巢癌治疗的一个有价值的靶点。
