Amitina N N, Ginevskaia V A, Shirman G A, Baeva L E, Ivannikova T I
Vopr Virusol. 1988 May-Jun;33(3):319-23.
The use of immunoperoxidase method for detection of virus antigens in a fixed infected cell monolayer on microplates (cellular immunoperoxidase method, CIPM) for poliomyelitis and hepatitis A virus as well as for human and simian SA II rotaviruses is described, as well as the method of quantitation of rotaviruses by counting of virus multiplication foci. The CIPM combines the advantages of the methods based on the infectivity determinations and of immunochemical methods, and may be used both for diagnosis and for detection and quantitation of enteric viruses.
本文描述了使用免疫过氧化物酶法在微孔板上固定感染的细胞单层中检测脊髓灰质炎病毒、甲型肝炎病毒以及人和猿猴SA II轮状病毒的病毒抗原(细胞免疫过氧化物酶法,CIPM),以及通过计数病毒增殖灶来定量轮状病毒的方法。CIPM结合了基于感染性测定的方法和免疫化学方法的优点,可用于肠道病毒的诊断、检测和定量。